Profiling Serum Cytokines and Anticytokine Antibodies in Psoriasis Patients.
Profiling Serum Cytokines and Anticytokine Antibodies in Psoriasis Patients.
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银屑病患者的血清细胞因子和抗细胞因子抗体分析
DOI：
10.1155/2022/2787954
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发表时间：
2022
影响因子：
4.1
通讯作者：
Wang, Liangchun
中科院分区：
文献类型：
--
作者：
Hong, Dan;Liu, Xiuting;Qiu, Xiaonan;Lu, Siyao;Jiang, Yanyun;Tan, Guozhen;Shi, Zhenrui;Wang, Liangchun
已经一致地发现细胞因子如IL-17 A在银肩病病变皮肤中升高，并且针对IL-17的治疗性抗体已经证明在治疗银肩病皮肤和关节疾病中的功效。然而，有关银屑病患者循环细胞因子的结果仍存在争议。抗细胞因子自身抗体（ACAAs）在各种自身免疫性疾病中被检测到，但在银屑病中仍然很大程度上未知。我们的目的是调查银屑病患者血清细胞因子和ACAAs的水平。该研究包括44名未经生物制剂治疗的银屑病患者和40名健康对照。血清细胞因子和相应的自身抗体的测定采用多重微球技术。通过原代角质形成细胞中IL-8的产生来测定血清IL-17 A的生物活性。在此，我们发现银屑病患者的IL-12 B（中位数：6.16对9.03，p = 0.0194）和Th 17细胞因子（IL-17 A：中位数：0.32对1.05，p = 0.0026; IL-22：中位数：4.41对4.41，p = 0.0120）的血清水平升高。更有趣的是，在一定比例的患者中鉴定出生物活性IL-17 A，并且与疾病严重程度呈正相关。在银屑病中，多种细胞因子相互之间存在着密切的联系，形成了一个独特的组群。在13种抗细胞因子抗体中，银屑病患者的抗IL-22抗体中度低于对照组（中位数：262.8 vs.190.5，p = 0.0418），抗IL-15抗体略高于对照组（中位数：25.5 vs.30.5，p = 0.0069）。ACAAs与疾病严重程度无关。因此，抗体与细胞因子的比率随细胞因子的模式而变化。总之，我们的研究结果表明，循环生物活性IL-17 A的水平与银屑病患者的疾病活动性相关。与此相反，ACA的滴度没有显着改变，也不与疾病的严重程度。然而，ACAAs的功能性仍有待于在未来的研究中进一步证明。
Cytokines like IL-17A have been consistently found to be elevated in psoriatic lesional skin, and therapeutic antibodies to IL-17 have demonstrated efficacy in treating psoriatic skin and joint disease. However, results about the circulating cytokines in psoriasis patients remained controversial. Anticytokine autoantibodies (ACAAs) were detected in various autoimmune diseases but remained largely unknown in psoriasis. We aimed to investigate the serum levels of cytokines and ACAAs in psoriasis patients. The study included 44 biologics-naive psoriasis patients and 40 healthy controls. Serum cytokines and the corresponding autoantibodies were measured by multiplex bead-based technology. The bioactivity of serum IL-17A was determined by IL-8 production in primary keratinocytes. Herein, we found serum levels of IL-12B (median: 6.16 vs. 9.03, p = 0.0194) and Th17 cytokines (IL-17A: median: 0.32 vs. 1.05, p = 0.0026; IL-22: median: 4.41 vs. 4.41, p = 0.0120) were increased in psoriasis patients. More interestingly, bioactive IL-17A was identified in a proportion of patients and positively correlated with disease severity. A few of cytokines were closely associated with each other and formed into a distinct panel in psoriasis. Of 13 anticytokine antibodies, anti-IL-22 was moderately lower (median: 262.8 vs.190.5, p = 0.0418), and anti-IL-15 was slightly higher (median: 25.5 vs. 30.5, p = 0.0069) in psoriasis than controls. None of ACAAs was related to disease severity. Consequently, the ratios of antibodies to cytokines varied with the pattern of cytokines. In summary, our finding suggested that the levels of circulating bioactive IL-17A were associated with disease activity in psoriasis patients. In contrast, the titers of ACAAs were not significantly altered nor correlated with disease severity. However, the functionality of ACAAs remains to be further demonstrated in vitro in future studies.
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影响因子：
10.3
作者：
Coimbra, S.;Oliveira, H.;Santos-Silva, A.
通讯作者：
Santos-Silva, A.
DOI：
10.1038/nrdp.2016.82
发表时间：
2016-11-24
期刊：
Nature reviews. Disease primers
影响因子：
--
作者：
Greb, Jacqueline E;Goldminz, Ari M;Gottlieb, Alice B
通讯作者：
Gottlieb, Alice B
影响因子：
158.5
作者：
Langley, Richard G.;Elewski, Boni E.;Papavassilis, Charis
通讯作者：
Papavassilis, Charis
影响因子：
4.6
作者：
Bergman, R.;Ramon, M.;Karin, N.
通讯作者：
Karin, N.
影响因子：
7.3
作者：
Brembilla NC;Senra L;Boehncke WH
通讯作者：
Boehncke WH