Genome-Wide Study of Hsp90 Gene Family in Cabbage (Brassica oleracea var. capitata L.) and Their Imperative Roles in Response to Cold Stress.
Genome-Wide Study of Hsp90 Gene Family in Cabbage (Brassica oleracea var. capitata L.) and Their Imperative Roles in Response to Cold Stress.
复制标题
卷心菜（Brassica oleracea var.capitala L.）Hsp90 基因家族的全基因组研究及其在应对冷胁迫中的重要作用。
DOI：
10.3389/fpls.2022.908511
复制
发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
热休克蛋白90（Hsp90）在植物发育调节和防御反应中发挥着重要作用。已经对几种植物物种的 Hsp90 家族基因进行了检查。然而，迄今为止，卷心菜中的 Hsp90 基因家族尚未得到充分研究。在这项研究中，我们在卷心菜（Brassica oleracea var.capitala L.）中发现了 12 个 BoHsp90 基因。根据系统发育分析，这些甘蓝 Hsp90 基因被分为五组。在这五个组中，第一组包含五个 Hsp90 基因，包括 BoHsp90-1、BoHsp90-2、BoHsp90-6、BoHsp90-10 和 BoHsp90-12。第二组包含三个 Hsp90 基因，包括 BoHsp90-3、BoHsp90-4 和 BoHsp90。第三组仅包含一个Hsp90基因，包括BoHsp90-9。第四组由三个Hsp90基因组成，包括BoHsp90-5、BoHsp90-7和BoHsp90-8，第五组中没有来自甘蓝的Hsp90基因。同线性分析表明，共有12个BoHsp90基因与5个拟南芥基因和10个白菜基因存在共线性关系。启动子评估表明甘蓝Hsp90基因的启动子包含环境胁迫相关和激素响应的顺式元件。 RNA-seq数据分析表明BoHsp90-9和BoHsp90-5的组织特异性表达在茎、叶、长角果和花中高表达。此外，甘蓝BoHsp90的表达模式显示BoHsp90-2、BoHsp90-3、BoHsp90-7、BoHsp90-9、BoHsp90-10和BoHsp90-11在冷胁迫下被诱导，这表明这些Hsp90基因在冷驯化中发挥着重要作用，并支持正常生长和发育过程的持续。甘蓝 Hsp90 基因家族被发现在应对冷胁迫时存在差异表达，表明这些基因在寒冷条件下甘蓝生长发育中发挥着重要作用。
Heat shock protein 90 (Hsp90) plays an important role in plant developmental regulation and defensive reactions. Several plant species have been examined for the Hsp90 family gene. However, the Hsp90 gene family in cabbage has not been well investigated to date. In this study, we have been discovered 12 BoHsp90 genes in cabbage (Brassica oleracea var. capitata L.). These B. oleracea Hsp90 genes were classified into five groups based on phylogenetic analysis. Among the five groups, group one contains five Hsp90 genes, including BoHsp90-1, BoHsp90-2, BoHsp90-6, BoHsp90-10, and BoHsp90-12. Group two contains three Hsp90 genes, including BoHsp90-3, BoHsp90-4, and BoHsp90. Group three only includes one Hsp90 gene, including BoHsp90-9. Group four were consisting of three Hsp90 genes including BoHsp90-5, BoHsp90-7, and BoHsp90-8, and there is no Hsp90 gene from B. oleracea in the fifth group. Synteny analysis showed that a total of 12 BoHsp90 genes have a collinearity relationship with 5 Arabidopsis genes and 10 Brassica rapa genes. The promoter evaluation revealed that the promoters of B. oleracea Hsp90 genes included environmental stress-related and hormone-responsive cis-elements. RNA-seq data analysis indicates that tissue-specific expression of BoHsp90-9 and BoHsp90-5 were highly expressed in stems, leaves, silique, and flowers. Furthermore, the expression pattern of B. oleracea BoHsp90 exhibited that BoHsp90-2, BoHsp90-3, BoHsp90-7, BoHsp90-9, BoHsp90-10, and BoHsp90-11 were induced under cold stress, which indicates these Hsp90 genes perform a vital role in cold acclimation and supports in the continual of normal growth and development process. The cabbage Hsp90 gene family was found to be differentially expressed in response to cold stress, suggesting that these genes play an important role in cabbage growth and development under cold conditions.
登录
查看更多内容
影响因子：
3.3
作者：
Huang Z;Duan W;Song X;Tang J;Wu P;Zhang B;Hou X
通讯作者：
Hou X
影响因子：
5.2
作者：
Hu, Wenhuo;Hu, Guocheng;Han, Bin
通讯作者：
Han, Bin
影响因子：
14.9
作者：
Goodstein DM;Shu S;Howson R;Neupane R;Hayes RD;Fazo J;Mitros T;Dirks W;Hellsten U;Putnam N;Rokhsar DS
通讯作者：
Rokhsar DS
影响因子：
10.1
作者：
Gu, Lianhong;Hanson, Paul J.;Meyers, Tilden
通讯作者：
Meyers, Tilden
影响因子：
5.6
作者：
Hassan MA;Xiang C;Farooq M;Muhammad N;Yan Z;Hui X;Yuanyuan K;Bruno AK;Lele Z;Jincai L
通讯作者：
Jincai L