Fine-scale mapping of natural variation in fly fecundity identifies neuronal domain of expression and function of an aquaporin.
Fine-scale mapping of natural variation in fly fecundity identifies neuronal domain of expression and function of an aquaporin.
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DOI：
10.1371/journal.pgen.1002631
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发表时间：
2012
期刊：
影响因子：
4.5
通讯作者：
Tatar M
中科院分区：
文献类型：
--
作者：
Bergland AO;Chae HS;Kim YJ;Tatar M
为了深入了解在健身相关性状的常设变化的分子遗传基础，我们确定了一个新的因素，调节雌性果蝇繁殖力的自然变异的分子和生理基础。来自野生果园种群的果蝇雌性生殖力的遗传变异是可遗传的，并且在很大程度上独立于其他测量的生活史特征。我们将这种变异的一部分定位到一个QTL上，然后使用缺陷作图将这个QTL进一步细化到5个候选基因。仅针对这些基因中的一个基因（由Drip编码的水通道蛋白）的RNAi的普遍表达降低了繁殖力。在我们的映射人群中，头部而不是其他组织中的Drip mRNA水平与繁殖力呈正相关。我们本地化滴水表达的一小群corazonin生产神经元位于前脑的背外侧后室。使用泛神经元ELAV-Gal 4和Crz-Gal 4驱动物的Drip-RNAi表达降低了繁殖力。低生育力RILs降低Crz表达和增加pale表达，pale是编码昆虫生活史调节剂多巴胺生产中限速步骤的酶。总之，这些数据表明，自然变化的滴在corazonin生产神经元的表达，有助于通过改变两种神经激素的浓度在健身的常设变化。现代进化生物学的一个主要目标是阐明适合度性状的遗传基础。这一目标对于全面了解进化过程非常重要，因为它使我们能够了解适应性性状的自然选择模式，并确定影响适应性性状的分子和生理过程。在这里，我们描述了我们的工作，以确定自然变异的分子遗传和生理基础的核心生活史特征，繁殖力，果蝇。使用各种映射技术，我们表明，差异表达的水通道蛋白滴在神经组织中影响女性生育力的自然变化。我们进一步描述了产生昆虫应激激素corazonin的神经元中Drop的一个新表达域，并证明这些神经元中Drop的差异表达通过调节corazonin和多巴胺的浓度来影响雌性生殖力。这一令人惊讶和新颖的观察突出了利用自然遗传变异来识别表型性状背后的分子过程的好处。
To gain insight into the molecular genetic basis of standing variation in fitness related traits, we identify a novel factor that regulates the molecular and physiological basis of natural variation in female Drosophila melanogaster fecundity. Genetic variation in female fecundity in flies derived from a wild orchard population is heritable and largely independent of other measured life history traits. We map a portion of this variation to a single QTL and then use deficiency mapping to further refine this QTL to 5 candidate genes. Ubiquitous expression of RNAi against only one of these genes, an aquaporin encoded by Drip, reduces fecundity. Within our mapping population Drip mRNA level in the head, but not other tissues, is positively correlated with fecundity. We localize Drip expression to a small population of corazonin producing neurons located in the dorsolateral posterior compartments of the protocerebrum. Expression of Drip–RNAi using both the pan-neuronal ELAV-Gal4 and the Crz-Gal4 drivers reduces fecundity. Low-fecundity RILs have decreased Crz expression and increased expression of pale, the enzyme encoding the rate-limiting step in the production of dopamine, a modulator of insect life histories. Taken together these data suggest that natural variation in Drip expression in the corazonin producing neurons contributes to standing variation in fitness by altering the concentration of two neurohormones. A major goal of modern evolutionary biology is to elucidate the genetic basis of standing genetic variation underlying fitness traits. This goal is important for a comprehensive picture of the evolutionary process, because it allows us to understand the mode of natural selection on fitness traits and identify the molecular and physiological processes that affect fitness traits. Here, we describe our work to identify the molecular genetic and physiological basis for natural variation in a core life history trait, fecundity, of Drosophila melanogaster. Using a variety of mapping techniques, we show that differential expression of the aquaporin Drip in nervous tissue affects natural variation in female fecundity. We further go on to describe a novel domain of expression of Drip in neurons that produce the insect stress hormone corazonin and demonstrate that differential expression of Drip in these neurons affects female fecundity putatively through modulating the concentration of corazonin and dopamine. This surprising and novel observation highlights the benefit of exploiting natural genetic variation to identify the molecular processes underlying phenotypic traits.
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影响因子：
2.2
作者：
Gruntenko, NE;Karpova, EK;Rauschenbach, IY
通讯作者：
Rauschenbach, IY
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作者：
Gautam, Naveen-Kumar;Tapadia, Madhu G.
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3.3
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Service, PM
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作者：
Kingsolver, JG;Gomulkiewicz, R;Carter, PA
通讯作者：
Carter, PA
影响因子：
56.9
作者：
Johanson, U;West, J;Dean, C
通讯作者：
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