Comparison of host immune responses to homologous and heterologous type II porcine reproductive and respiratory syndrome virus (PRRSV) challenge in vaccinated and unvaccinated pigs.
Comparison of host immune responses to homologous and heterologous type II porcine reproductive and respiratory syndrome virus (PRRSV) challenge in vaccinated and unvaccinated pigs.
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DOI：
10.1155/2014/416727
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发表时间：
2014
影响因子：
--
通讯作者：
Shi J
中科院分区：
文献类型：
--
作者：
Li X;Galliher-Beckley A;Pappan L;Trible B;Kerrigan M;Beck A;Hesse R;Blecha F;Nietfeld JC;Rowland RR;Shi J
猪繁殖与呼吸综合征（PRRS）是一种严重的动物疾病，由于田间PRRS病毒的高度遗传变异，目前的疫苗对感染的保护作用有限。因此，了解不同的PRRSV毒株引起的宿主免疫应答将有助于开发更有效的疫苗。使用IngelVac修饰的活PRRSV疫苗（MLV）、其亲本毒株VR-2332和异源KS-06-72109毒株（PRRSV的堪萨斯分离株），我们比较了由疫苗接种和/或PRRSV感染诱导的免疫应答。我们的结果表明，MLV可以提供对同源病毒（VR-2332）的完全保护和对异源病毒（KS-06）攻击的部分保护。保护与攻击时的PRRSV中和抗体水平相关，与KS-06株相比，接种疫苗的猪对VR-2332具有更高的滴度。攻毒株未改变免疫猪血清或T细胞亚群中的细胞因子表达谱。然而，当PBMC用PRRSV再刺激时，在回忆应答中用异源PRRSV攻击的猪产生更高频率的分泌IFN-γ的PBMC。因此，这项研究表明，血清中和抗体滴度与PRRSV疫苗诱导的保护同源和异源的挑战。
Porcine reproductive and respiratory syndrome (PRRS) is a high-consequence animal disease with current vaccines providing limited protection from infection due to the high degree of genetic variation of field PRRS virus. Therefore, understanding host immune responses elicited by different PRRSV strains will facilitate the development of more effective vaccines. Using IngelVac modified live PRRSV vaccine (MLV), its parental strain VR-2332, and the heterologous KS-06-72109 strain (a Kansas isolate of PRRSV), we compared immune responses induced by vaccination and/or PRRSV infection. Our results showed that MLV can provide complete protection from homologous virus (VR-2332) and partial protection from heterologous (KS-06) challenge. The protection was associated with the levels of PRRSV neutralizing antibodies at the time of challenge, with vaccinated pigs having higher titers to VR-2332 compared to KS-06 strain. Challenge strain did not alter the cytokine expression profiles in the serum of vaccinated pigs or subpopulations of T cells. However, higher frequencies of IFN-γ-secreting PBMCs were generated from pigs challenged with heterologous PRRSV in a recall response when PBMCs were re-stimulated with PRRSV. Thus, this study indicates that serum neutralizing antibody titers are associated with PRRSV vaccination-induced protection against homologous and heterologous challenge.
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影响因子：
5.5
作者：
Li, Xiangdong;Galliher-Beckley, Amy;Huang, Hongzhou;Sun, Xiuzhi;Shi, Jishu
通讯作者：
Shi, Jishu
影响因子：
3.3
作者：
Meng XJ
通讯作者：
Meng XJ
影响因子：
3.7
作者：
Guo B;Lager KM;Henningson JN;Miller LC;Schlink SN;Kappes MA;Kehrli ME Jr;Brockmeier SL;Nicholson TL;Yang HC;Faaberg KS
通讯作者：
Faaberg KS
影响因子：
5.5
作者：
Lawson, Steven;Lunney, Joan;Christopher-Hennings, Jane
通讯作者：
Christopher-Hennings, Jane
影响因子：
3.7
作者：
Osorio, FA;Galeota, JA;Laegreid, WW
通讯作者：
Laegreid, WW