A fate map of the murine pancreas buds reveals a multipotent ventral foregut organ progenitor.
A fate map of the murine pancreas buds reveals a multipotent ventral foregut organ progenitor.
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DOI：
10.1371/journal.pone.0040707
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Tremblay KD
中科院分区：
文献类型：
--
作者：
Angelo JR;Guerrero-Zayas MI;Tremblay KD
定形内胚层是胚胎胚层，它产生出芽的内胚层器官，包括甲状腺、肺、肝脏和胰腺以及肠管的其余部分。 DiI命运图谱和全胚胎培养用于确定交配后9.5天（dpc）背侧和腹侧胰腺芽的内胚层起源。我们的结果表明，每个芽的祖细胞占据不同的内胚层区域。背芽祖细胞位于第 2 和第 11 体节阶段 (SS) 之间的第 2-4 体节上方的内侧内胚层中。形成腹侧胰芽的内胚层存在于两个不同的区域。第一个区域源自左侧和右侧内胚层尾部，通过 6 SS 到达前肠门，第二个区域源自内胚层唇腹侧中线 (VMEL)。与位于侧面的腹侧前肠祖细胞不同，VMEL群体具有多能祖细胞，该祖细胞以时间限制的方式促进甲状腺芽、肝芽的头帽、肝芽的腹侧中线和腹侧胰腺芽的中线。该数据表明 9.5 dpc 甲状腺、肝脏和腹侧胰腺芽的中线源自相同的祖细胞群，证明所有三个腹侧前肠芽之间的发育联系。总而言之，这些数据定义了预先指定的内胚层片中背侧和腹侧胰腺祖细胞的位置，并且应该有助于深入了解胰腺规范所需的诱导事件。
The definitive endoderm is the embryonic germ layer that gives rise to the budding endodermal organs including the thyroid, lung, liver and pancreas as well as the remainder of the gut tube. DiI fate mapping and whole embryo culture were used to determine the endodermal origin of the 9.5 days post coitum (dpc) dorsal and ventral pancreas buds. Our results demonstrate that the progenitors of each bud occupy distinct endodermal territories. Dorsal bud progenitors are located in the medial endoderm overlying somites 2–4 between the 2 and 11 somite stage (SS). The endoderm forming the ventral pancreas bud is found in 2 distinct regions. One territory originates from the left and right lateral endoderm caudal to the anterior intestinal portal by the 6 SS and the second domain is derived from the ventral midline of the endoderm lip (VMEL). Unlike the laterally located ventral foregut progenitors, the VMEL population harbors a multipotent progenitor that contributes to the thyroid bud, the rostral cap of the liver bud, ventral midline of the liver bud and the midline of the ventral pancreas bud in a temporally restricted manner. This data suggests that the midline of the 9.5 dpc thyroid, liver and ventral pancreas buds originates from the same progenitor population, demonstrating a developmental link between all three ventral foregut buds. Taken together, these data define the location of the dorsal and ventral pancreas progenitors in the prespecified endodermal sheet and should lead to insights into the inductive events required for pancreas specification.
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