asunder is required for dynein localization and dorsal fate determination during Drosophila oogenesis.
asunder is required for dynein localization and dorsal fate determination during Drosophila oogenesis.
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DOI：
10.1016/j.ydbio.2013.12.004
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发表时间：
2014-02-01
影响因子：
2.7
通讯作者：
Lee, Laura A.
中科院分区：
文献类型：
--
作者：
Sitaram, Poojitha;Merkle, Julie A.;Lee, Ethan;Lee, Laura A.
我们以前表明，asunder（asun）是果蝇精子发生过程中动力蛋白定位的关键调节因子。由于asun在果蝇卵巢和早期胚胎中的表达比在睾丸中高得多，因此我们在此试图确定ASUN是否在卵子发生和/或胚胎发生中起作用。我们的特点是女性生殖系表型的果蝇纯合子的空等位基因asun（asund 93）。我们发现，asund 93雌性产卵很少，包含一个高度混乱的卵巢管的安排与野生型女性相比，较小的卵巢。asund 93卵巢还含有大量具有结构缺陷的卵室。由asund 93雌性产下的大多数卵都有不同程度的腹侧化，从轻度到重度;这种腹侧化表型可能是继发于asund 93卵母细胞内gurken转录本的缺陷定位，这是一个动力蛋白调节的步骤。我们发现，动力蛋白定位是异常的asund 93卵母细胞，表明ASUN是需要在男性和女性生殖细胞的这一过程。除了gurken mRNA定位的损失，asund 93卵巢表现出缺陷，在其他动力蛋白介导的过程中，如迁移的护士细胞中心体进入卵母细胞在有丝分裂早期，维持卵母细胞核的前背区域的卵母细胞在晚期卵室，卵母细胞核和中心体之间的耦合。总之，我们的数据表明asun是果蝇卵子发生过程中动力蛋白定位和动力蛋白介导的过程的关键调节因子。
We previously showed that asunder (asun) is a critical regulator of dynein localization during Drosophila spermatogenesis. Because the expression of asun is much higher in Drosophila ovaries and early embryos than in testes, we herein sought to determine whether ASUN plays roles in oogenesis and/or embryogenesis. We characterized the female germline phenotypes of flies homozygous for a null allele of asun (asund93). We find that asund93 females lay very few eggs and contain smaller ovaries with a highly disorganized arrangement of ovarioles in comparison to wild-type females. asund93 ovaries also contain a significant number of egg chambers with structural defects. A majority of the eggs laid by asund93 females are ventralized to varying degrees, from mild to severe; this ventralization phenotype may be secondary to defective localization of gurken transcripts, a dynein-regulated step, within asund93 oocytes. We find that dynein localization is aberrant in asund93 oocytes, indicating that ASUN is required for this process in both male and female germ cells. In addition to the loss of gurken mRNA localization, asund93 ovaries exhibit defects in other dynein-mediated processes such as migration of nurse cell centrosomes into the oocyte during the early mitotic divisions, maintenance of the oocyte nucleus in the anterior-dorsal region of the oocyte in late-stage egg chambers, and coupling between the oocyte nucleus and centrosomes. Taken together, our data indicate that asun is a critical regulator of dynein localization and dynein-mediated processes during Drosophila oogenesis.
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LIS1和NDEL1会影响神经干细胞中核膜分解的时机。
影响因子：
7.8
作者：
Hebbar, Sachin;Mesngon, Mariano T.;Guillotte, Aimee M.;Desai, Bhavim;Ayala, Ramses;Smith, Deanna S.
通讯作者：
Smith, Deanna S.
影响因子：
4
作者：
Hook, Peter;Vallee, Richard B.
通讯作者：
Vallee, Richard B.
DOI：
10.1083/jcb.126.6.1475
发表时间：
1994-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Li M;McGrail M;Serr M;Hays TS
通讯作者：
Hays TS
影响因子：
9.2
作者：
Bastock, Rebecca;St Johnston, Daniel
通讯作者：
St Johnston, Daniel
DOI：
10.1002/dvg.1020160104
发表时间：
1995-01-01
期刊：
DEVELOPMENTAL GENETICS
影响因子：
--
作者：
LIN, HF;SPRADLING, AC
通讯作者：
SPRADLING, AC