Mussel-Inspired Multifunctional Hydrogel Coating for Prevention of Infections and Enhanced Osteogenesis.
Mussel-Inspired Multifunctional Hydrogel Coating for Prevention of Infections and Enhanced Osteogenesis.
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DOI：
10.1021/acsami.6b16779
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发表时间：
2017-04-05
影响因子：
9.5
通讯作者：
Khademhosseini A
中科院分区：
文献类型：
--
作者：
Cheng H;Yue K;Kazemzadeh-Narbat M;Liu Y;Khalilpour A;Li B;Zhang YS;Annabi N;Khademhosseini A
预防术后感染和促进生物整合是骨科植入物获得长期成功的关键因素。通过植入物表面局部递送抗生素和生物活性分子是实现这些目标的有希望的方法。然而，先前报道的用于钛合金植入物的表面功能化以负载生物活性成分的方法遭受耗时的复杂过程和缺乏长期稳定性。在这里，我们介绍了钛植入物的粘合剂，骨传导和抗菌水凝胶涂层的设计和表征。为了形成这种多功能水凝胶，用邻苯二酚基序对基于光交联明胶的水凝胶进行改性，以增强对Ti表面的粘附，从而促进涂层稳定性。为了诱导抗微生物和骨传导特性，将短阳离子抗微生物肽（AMP）和合成硅酸盐纳米颗粒（SN）引入水凝胶制剂中。水凝胶中AMP的控制释放显示出优异的抗微生物活性，以防止生物膜形成。此外，当与人类间充质干细胞一起培养时，向水凝胶制剂中添加SN增强了骨生成，这表明有可能促进周围组织中的新骨形成。考虑到我们的植入物水凝胶涂层的独特特征，包括高粘附性、抗微生物能力和诱导成骨的能力，我们认为我们的设计为骨植入物表面改性和功能化提供了一种有用的替代方法。
Prevention of postsurgery infection and promotion of biointegration are the key factors to achieve long-term success in orthopedic implants. Localized delivery of antibiotics and bioactive molecules by the implant surface serves as a promising approach toward these goals. However, previously reported methods for surface functionalization of the titanium alloy implants to load bioactive ingredients suffer from time-consuming complex processes and lack of long-term stability. Here, we present the design and characterization of an adhesive, osteoconductive, and antimicrobial hydrogel coating for Ti implants. To form this multifunctional hydrogel, a photo-cross-linkable gelatin-based hydrogel was modified with catechol motifs to enhance adhesion to Ti surfaces and thus promote coating stability. To induce antimicrobial and osteoconductive properties, a short cationic antimicrobial peptide (AMP) and synthetic silicate nanoparticles (SNs) were introduced into the hydrogel formulation. The controlled release of AMP loaded in the hydrogel demonstrated excellent antimicrobial activity to prevent biofilm formation. Moreover, the addition of SNs to the hydrogel formulation enhanced osteogenesis when cultured with human mesenchymal stem cells, suggesting the potential to promote new bone formation in the surrounding tissues. Considering the unique features of our implant hydrogel coating, including high adhesion, antimicrobial capability, and the ability to induce osteogenesis, it is believed that our design provides a useful alternative method for bone implant surface modification and functionalization.
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DOI：
10.1002/adma.201503255
发表时间：
2016-01-06
期刊：
Advanced materials (Deerfield Beach, Fla.)
影响因子：
--
作者：
Annabi N;Shin SR;Tamayol A;Miscuglio M;Bakooshli MA;Assmann A;Mostafalu P;Sun JY;Mithieux S;Cheung L;Tang XS;Weiss AS;Khademhosseini A
通讯作者：
Khademhosseini A
影响因子：
29.4
作者：
Gaharwar, Akhilesh K.;Mihaila, Silvia M.;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
DOI：
10.1007/s10856-010-4035-3
发表时间：
2010-06-01
影响因子：
3.7
作者：
Lai, Jui-Yang
通讯作者：
Lai, Jui-Yang
影响因子：
9.7
作者：
Cheng, Hao;Xiong, Wei;Li, Feng
通讯作者：
Li, Feng
影响因子：
14
作者：
Kazemzadeh-Narbat, Mehdi;Kindrachuk, Jason;Wang, Rizhi
通讯作者：
Wang, Rizhi