Ion-Selective Nanosensor for Photoacoustic and Fluorescence Imaging of Potassium.
Ion-Selective Nanosensor for Photoacoustic and Fluorescence Imaging of Potassium.
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DOI：
10.1021/acs.analchem.7b00930
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发表时间：
2017-08-01
影响因子：
7.4
通讯作者：
Kopelman R
中科院分区：
文献类型：
--
作者：
Lee CH;Folz J;Zhang W;Jo J;Tan JWY;Wang X;Kopelman R
离子选择性光极 (ISO) 是离子选择性电极的光学模拟，在化学和生化分析中发挥着越来越重要的作用。在这里，我们将该技术扩展到离子选择性光声光极 (ISPAO)，它同时用作基于荧光的 ISO，并将其专门应用于钾 (K+)。值得注意的是，钾离子是生物系统中最丰富的阳离子之一，参与许多生理和病理过程。此外，最近有报道称，肿瘤中异常细胞外钾浓度的存在会抑制免疫反应，从而抑制免疫治疗。然而，不幸的是，能够提供体内钾图像的传感器仍然是一个未来的提议。在这里，我们制备了一种离子选择性钾纳米传感器（NS），旨在对细胞外环境进行体内光声（PA）化学成像，同时也能够进行基于荧光的细胞内离子选择性成像。这种钾纳米传感器 (K+ NS) 根据钾浓度调节其光学特性（吸光度和荧光）。 K+ NS 能够通过基于 ISPAO 的测量来测量 1 mM 至 100 mM 范围内的钾，具有高灵敏度和选择性。此外，近红外染料表面改性的 K+ NS 允许在 20 mM 至 1 M 范围内进行基于荧光的钾传感。因此，K+ NS 可用作 PA 和基于荧光的纳米传感器，响应跨生物相关的 K+ 浓度，从细胞外 5 mM 典型值（通过 PA 成像）到细胞内 150 mM 典型值（通过荧光成像）。
Ion-selective optodes (ISOs), the optical analog of ion-selective electrodes, have played an increasingly important role in chemical and biochemical analysis. Here we extend this technique to ion-selective photoacoustic optodes (ISPAOs) that serve at the same time as fluorescence-based ISOs, and apply it specifically to potassium (K+). Notably, the potassium ion is one of the most abundant cations in biological systems, involved in numerous physiological and pathological processes. Furthermore, it has been recently reported that the presence of abnormal extracellular potassium concentrations in tumors suppresses the immune responses and thus suppresses immunotherapy. However, unfortunately, sensors capable of providing potassium images in vivo are still a future proposition. Here, we prepared an ion-selective potassium nanosensor (NS) aimed at in vivo photoacoustic (PA) chemical imaging of the extracellular environment, while being also capable of fluorescence based intracellular ion-selective imaging. This potassium nanosensor (K+ NS) modulates its optical properties (absorbance and fluorescence) according to the potassium concentration. The K+ NS is capable of measuring potassium, in the range of 1 mM to 100 mM, with high sensitivity and selectivity, by ISPAO based measurements. Also, a near infrared dye surface modified K+ NS allows fluorescence-based potassium sensing in the range of 20 mM to 1 M. The K+ NS serves thus as both PA and fluorescence based nanosensor, with response across the biologically relevant K+ concentrations, from the extracellular 5 mM typical values (through PA imaging) to the intracellular 150 mM typical values (through fluorescence imaging).
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DOI：
10.1039/c3an00093a
发表时间：
2013-06-07
期刊：
The Analyst
影响因子：
--
作者：
Ray A;Yoon HK;Koo Lee YE;Kopelman R;Wang X
通讯作者：
Wang X
影响因子：
15
作者：
Dubach, J. Matthew;Harjes, Daniel I.;Clark, Heather A.
通讯作者：
Clark, Heather A.
影响因子：
4.2
作者：
Sumner, JP;Kopelman, R
通讯作者：
Kopelman, R
DOI：
10.1146/annurev-anchem-071213-020307
发表时间：
2014-01-01
期刊：
ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 7
影响因子：
--
作者：
Mistlberger, Guenter;Crespo, Gaston A.;Bakker, Eric
通讯作者：
Bakker, Eric
影响因子：
7.4
作者：
Barker, SLR;Shortreed, MR;Kopelman, R
通讯作者：
Kopelman, R