Metabo-tip: a metabolomics platform for lifestyle monitoring supporting the development of novel strategies in predictive, preventive and personalised medicine.
Metabo-tip: a metabolomics platform for lifestyle monitoring supporting the development of novel strategies in predictive, preventive and personalised medicine.
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DOI：
10.1007/s13167-021-00241-6
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发表时间：
2021-06
期刊：
影响因子：
--
通讯作者：
Gerner C
中科院分区：
文献类型：
--
作者：
Brunmair J;Bileck A;Stimpfl T;Raible F;Del Favero G;Meier-Menches SM;Gerner C
暴露于来自营养、药物、环境污染物或其他生活习惯的生物活性化合物可能会影响人体组织。为了深入了解这些影响的影响，以及它们背后的基本生化机制，个体分子谱分析似乎是一个很有前途的工具，可以支持预测性，预防性和个性化医学的进一步发展。我们开发了一种称为代谢尖端的分析方法，用于分析从指尖收集的汗液，使用质谱法，这是迄今为止最全面和灵敏的分析方法。为了评估这种检测方法，我们使用标准化的方案将志愿者暴露于各种外源性物质，并研究他们的代谢反应。早在饮用一杯咖啡、50克黑巧克力或一份柑橘类水果后15分钟，就观察到指尖汗液成分的显著变化，提供了关于摄入物质的相关信息。这不仅包括促进健康的生物活性化合物，还包括潜在的危险物质。此外，从口服摄入的药物（如安乃近）中鉴定代谢物表明该测定法适用于以个性化方式观察特定的酶促过程。值得注意的是，我们发现汗液成分以昼夜节律波动，支持汗液成分可以受到内源性代谢活动影响的假设。这一点进一步得到了过敏反应个体的代谢尖端试验中组胺显著增加的结果的证实。由于其分析能力、非侵入性特征和频繁采样的潜力，代谢尖端分析可能具有大量的实际应用，特别是关于特定生活方式和影响因素的个体化监测。metabo-tip提供的异常丰富的个体化代谢组学数据提供了对个体代谢活动的直接访问，因此将支持预测性预防性个性化医学。在线版本包含补充材料，可通过10.1007/s13167-021-00241-6获得。
Exposure to bioactive compounds from nutrition, pharmaceuticals, environmental contaminants or other lifestyle habits may affect the human organism. To gain insight into the effects of these influences, as well as the fundamental biochemical mechanisms behind them, individual molecular profiling seems to be a promising tool and may support the further development of predictive, preventive and personalised medicine. We developed an assay, called metabo-tip for the analysis of sweat, collected from fingertips, using mass spectrometry—by far the most comprehensive and sensitive method for such analyses. To evaluate this assay, we exposed volunteers to various xenobiotics using standardised protocols and investigated their metabolic response. As early as 15 min after the consumption of a cup of coffee, 50 g of dark chocolate or a serving of citrus fruits, significant changes in the sweat composition of the fingertips were observed, providing relevant information in regard to the ingested substances. This included not only health-promoting bioactive compounds but also potential hazardous substances. Furthermore, the identification of metabolites from orally ingested medications such as metamizole indicated the applicability of this assay to observe specific enzymatic processes in a personalised fashion. Remarkably, we found that the sweat composition fluctuated in a diurnal rhythm, supporting the hypothesis that the composition of sweat can be influenced by endogenous metabolic activities. This was further corroborated by the finding that histamine was significantly increased in the metabo-tip assay in individuals with allergic reactions. Metabo-tip analysis may have a large number of practical applications due to its analytical power, non-invasive character and the potential of frequent sampling, especially regarding the individualised monitoring of specific lifestyle and influencing factors. The extraordinarily rich individualised metabolomics data provided by metabo-tip offer direct access to individual metabolic activities and will thus support predictive preventive personalised medicine. The online version contains supplementary material available at 10.1007/s13167-021-00241-6.
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影响因子：
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作者：
Di Daniele N;Noce A;Vidiri MF;Moriconi E;Marrone G;Annicchiarico-Petruzzelli M;D'Urso G;Tesauro M;Rovella V;De Lorenzo A
通讯作者：
De Lorenzo A
DOI：
10.1039/c5an02387d
发表时间：
2016-03-21
期刊：
The Analyst
影响因子：
--
作者：
Jia M;Chew WM;Feinstein Y;Skeath P;Sternberg EM
通讯作者：
Sternberg EM
影响因子：
2
作者：
Adewole, Olanisun Olufemi;Erhabor, Greg Efosa;Prenni, Jessica E.
通讯作者：
Prenni, Jessica E.
DOI：
10.1002/prca.201800010
发表时间：
2018-11
期刊：
Proteomics. Clinical applications
影响因子：
--
作者：
Katchman BA;Zhu M;Blain Christen J;Anderson KS
通讯作者：
Anderson KS
DOI：
10.1016/j.jchromb.2019.121763
发表时间：
2019-09-15
影响因子：
3
作者：
Harshman, Sean W.;Pitsch, Rhonda L.;Martin, Jennifer A.
通讯作者：
Martin, Jennifer A.