Transcriptomics analysis and fed-batch regulation of high astaxanthin-producing Phaffia rhodozyma/Xanthophyllomyces dendrorhous obtained through adaptive laboratory evolution.
Transcriptomics analysis and fed-batch regulation of high astaxanthin-producing Phaffia rhodozyma/Xanthophyllomyces dendrorhous obtained through adaptive laboratory evolution.
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DOI：
10.1093/jimb/kuad015
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发表时间：
2023-02-17
影响因子：
3.4
通讯作者：
中科院分区：
文献类型：
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虾青素具有很强的抗氧化能力，在功能食品中具有很高的利用价值。然而，红酵母的虾青素含量相对较低。适应性实验室进化是获得高产菌株的极好方法。TiO 2是一种良好的氧化应激诱导剂。采用不同浓度的TiO 2对红酵母进行驯化培养，在1000 mg/L的TiO 2浓度下培养105 d，得到一株产虾青素的最佳菌株JMU-ALE 105。经发酵后，虾青素含量达到6.50 mg/g，比出发菌株提高了41.61%。ALE 105菌株经分批发酵和流加发酵，虾青素含量达到6.81mg/g。转录组学分析表明，ALE 105菌株的虾青素合成途径、脂肪酸、丙酮酸和氮代谢途径显著上调。基于氮代谢途径，采用硫酸铵流加发酵调节氮源，提高了虾青素含量，达到8.36mg/g。该研究为推进红酵母生产虾青素的产业化提供了技术基础和理论研究。通过TiO 2驯化获得一株虾青素高产菌株（ALE 105），并利用转录组学分析其代谢机制，结合氮源调控进一步提高虾青素产量。
Astaxanthin has high utilization value in functional food because of its strong antioxidant capacity. However, the astaxanthin content of Phaffia rhodozyma is relatively low. Adaptive laboratory evolution is an excellent method to obtain high-yield strains. TiO2 is a good inducer of oxidative stress. In this study, different concentrations of TiO2 were used to domesticate P. rhodozyma, and at a concentration of 1000 mg/L of TiO2 for 105 days, the optimal strain JMU-ALE105 for astaxanthin production was obtained. After fermentation, the astaxanthin content reached 6.50 mg/g, which was 41.61% higher than that of the original strain. The ALE105 strain was fermented by batch and fed-batch, and the astaxanthin content reached 6.81 mg/g. Transcriptomics analysis showed that the astaxanthin synthesis pathway, and fatty acid, pyruvate, and nitrogen metabolism pathway of the ALE105 strain were significantly upregulated. Based on the nitrogen metabolism pathway, the nitrogen source was adjusted by ammonium sulphate fed-batch fermentation, which increased the astaxanthin content, reaching 8.36 mg/g. This study provides a technical basis and theoretical research for promoting industrialization of astaxanthin production of P. rhodozyma. A high-yield astaxanthin strain (ALE105) was obtained through TiO2 domestication, and its metabolic mechanism was analysed by transcriptomics, which combined with nitrogen source regulation to further improve astaxanthin yield.
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影响因子：
29
作者：
Longo VD;Shadel GS;Kaeberlein M;Kennedy B
通讯作者：
Kennedy B
影响因子：
4.4
作者：
Cheng, Xin-Yi;Xiong, Yi-Jiao;Zhu, Ming-Jun
通讯作者：
Zhu, Ming-Jun
影响因子：
3.9
作者：
Angel Villegas-Mendez, Miguel;Papadaki, Aikaterini;Morales-Oyervides, Lourdes
通讯作者：
Morales-Oyervides, Lourdes
影响因子：
3
作者：
Almeida, E. L. M.;Ventorim, R. Z.;Silveira, W. B.
通讯作者：
Silveira, W. B.
影响因子：
5
作者：
Fu, Weiqi;Guomundsson, Olafur;Paglia, Giuseppe;Herjolfsson, Gisli;Andresson, Olafur S.;Palsson, Bernhard O.;Brynjolfsson, Sigurour
通讯作者：
Brynjolfsson, Sigurour