Genome-wide identification of bone metastasis-related microRNAs in lung adenocarcinoma by high-throughput sequencing.
Genome-wide identification of bone metastasis-related microRNAs in lung adenocarcinoma by high-throughput sequencing.
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通过高通量测序全基因组鉴定肺腺癌骨转移相关的 MicroRNA
DOI：
10.1371/journal.pone.0061212
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Xie L;Yang Z;Li G;Shen L;Xiang X;Liu X;Xu D;Xu L;Chen Y;Tian Z;Chen X
MicroRNAs(MiRNAs)是一类在转录后水平调节基因表达的非编码小RNA。它们参与多种生物学过程，包括细胞凋亡、增殖和转移。MiRNAs的异常表达已被发现在许多癌症中起重要作用。为了了解miRNAs在肺腺癌骨转移中的作用，我们从有骨转移和无骨转移的肺腺癌患者的血液中构建了两个小RNA文库。高通量测序结合差异表达分析发现，有骨转移的肺腺癌中有7个microRNAs表达下调，21个microRNAs表达上调。用生物信息学方法鉴定了797个差异表达的microRNAs的靶基因。功能注释分析表明，多条途径可能参与了肺腺癌的骨转移、原发灶存活和转移血管生成。这些信号通路包括MAPK、Wnt和NF-kappaB信号通路，以及涉及基质金属蛋白酶、细胞骨架蛋白和血管生成因子的通路。本研究对肺腺癌发生的分子机制提供了一些见解，从而有助于肺腺癌的诊断和治疗。
MicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression at the post-transcriptional level. They participate in a wide variety of biological processes, including apoptosis, proliferation and metastasis. The aberrant expression of miRNAs has been found to play an important role in many cancers. To understand the roles of miRNAs in the bone metastasis of lung adenocarcinoma, we constructed two small RNA libraries from blood of lung adenocarcinoma patients with and without bone metastasis. High-throughput sequencing combined with differential expression analysis identified that 7 microRNAs were down-regulated and 21 microRNAs were up-regulated in lung adenocarcinoma with bone metastasis. A total of 797 target genes of the differentially expressed microRNAs were identified using a bioinformatics approach. Functional annotation analysis indicated that a number of pathways might be involved in bone metastasis, survival of the primary origin and metastatic angiogenesis of lung adenocarcinoma. These include the MAPK, Wnt, and NF-kappaB signaling pathways, as well as pathways involving the matrix metalloproteinase, cytoskeletal protein and angiogenesis factors. This study provides some insights into the molecular mechanisms that underlie lung adenocarcinoma development, thereby aiding the diagnosis and treatment of the disease.
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