Quantitation of SPLUNC1 in saliva with an xMAP particle-based antibody capture and detection immunoassay.
Quantitation of SPLUNC1 in saliva with an xMAP particle-based antibody capture and detection immunoassay.
复制标题
用基于XMAP粒子的抗体捕获和检测免疫测定法中Splunc1的定量。
DOI：
10.1016/j.archoralbio.2011.08.013
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发表时间：
2012-02
影响因子：
3
通讯作者：
Brogiden, Kim A.
中科院分区：
文献类型：
--
作者：
Kohlgraf, Karl G.;Ackermann, Abbey R.;Burnell, Kindra K.;Srikantha, Rupasree N.;Joly, Sophie A.;Bartlett, Jennifer A.;Gakhar, Lokesh;Johnson, Georgia K.;McCray, Paul B., Jr.;Guthmiller, Janet M.;Brogiden, Kim A.
The short palate lung and nasal epithelial clone 1 (SPLUNC1) protein may be differentially expressed in oral infections, oral inflammatory disorders, or oral malignancies and may be involved in innate immune responses in the oral cavity. However, the actual concentration of SPLUNC1 in saliva has not previously been determined. In this study, we determined the concentrations of SPLUNC1 in saliva using a particle-based antibody capture and detection immunoassay. A commercial goat anti-rhSPLUNC1 polyclonal antibody (AF1897) was linked to fluorescent polystyrene microspheres and used as the capture antibody. A commercial mouse IgG2b anti-rhSPLUNC1 monoclonal antibody (MAB1897) was biotinylated and used as the detection antibody. Western blot and 2-dimensional fluorescence difference gel electrophoresis (2-D DIGE) analysis of immunoprecipitated rhSPLUNC1 and SPLUNC1 from saliva were used to show that the capture AF1897 and detection MAB1897 antibodies both recognized SPLUNC1. Protein concentrations in saliva from 20 subjects ranged from 0.9 to 23.9 mg/ml; SPLUNC1 concentrations ranged from 34.7 ng/ml to 13.8 μg/ml; and SPLUNC concentrations normalized per mg of total salivary protein ranged from 4.7 ng/ml to 5.3 μg/ml. These results show that SPLUNC1 is detected in saliva in a variety of concentrations. This immunoassay may prove to be useful in determining the concentration of SPLUNC1 in saliva for assessing its role in the pathogenesis of oral infections, oral inflammatory disorders, or oral malignancies.
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影响因子：
4.1
作者：
Mitas, M;Hoover, L;Gillanders, WE
通讯作者：
Gillanders, WE
影响因子：
7.3
作者：
Bingle, L;Cross, SS;Bingle, CD
通讯作者：
Bingle, CD
影响因子：
8.8
作者：
通讯作者：
--
影响因子：
3.5
作者：
Thunell DH;Tymkiw KD;Johnson GK;Joly S;Burnell KK;Cavanaugh JE;Brogden KA;Guthmiller JM
通讯作者：
Guthmiller JM
影响因子：
7.6
作者：
Ruhl, S;Rayment, SA;Troxler, RF
通讯作者：
Troxler, RF