Pain inhibition by optogenetic activation of specific anterior cingulate cortical neurons.
Pain inhibition by optogenetic activation of specific anterior cingulate cortical neurons.
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DOI：
10.1371/journal.pone.0117746
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Mohanty SK
中科院分区：
文献类型：
--
作者：
Gu L;Uhelski ML;Anand S;Romero-Ortega M;Kim YT;Fuchs PN;Mohanty SK
来自人类和动物的累积证据表明，前扣带皮层（ACC）对疼痛相关感知很重要，因此可能是疼痛缓解治疗的目标。然而，使用现有的基于电极的ACC刺激并没有显著减轻疼痛，至少部分原因是缺乏特异性，并且可能同时激活兴奋性和抑制性神经元。在此，我们报告了通过光遗传刺激ACC表达通道视紫红质-2的抑制性神经回路，转基因小鼠的疼痛行为显着减少。电生理测量证实，ACC抑制神经元的刺激与ACC神经活动的减少有关。此外，在ACC中观察到明显的光遗传刺激强度和频率依赖性抑制。此外，我们证实了丘脑中不同神经元单位对特定类型的疼痛刺激（如：疼痛刺激）的特定电生理反应。，福尔马林注射，捏)，我们发现这是由ACC抑制神经元的光遗传控制调节的。这些结果强调了ACC的抑制作为一种临床替代抑制慢性疼痛，并导致更好地理解扣带皮层的疼痛处理电路。
Cumulative evidence from both humans and animals suggests that the anterior cingulate cortex (ACC) is important for pain-related perception, and thus a likely target for pain relief therapy. However, use of existing electrode based ACC stimulation has not significantly reduced pain, at least in part due to the lack of specificity and likely co-activation of both excitatory and inhibitory neurons. Herein, we report a dramatic reduction of pain behavior in transgenic mice by optogenetic stimulation of the inhibitory neural circuitry of the ACC expressing channelrhodopsin-2. Electrophysiological measurements confirmed that stimulation of ACC inhibitory neurons is associated with decreased neural activity in the ACC. Further, a distinct optogenetic stimulation intensity and frequency-dependent inhibition of spiking activity in the ACC was observed. Moreover, we confirmed specific electrophysiological responses from different neuronal units in the thalamus, in response to particular types of painful stimuli (i,e., formalin injection, pinch), which we found to be modulated by optogenetic control of the ACC inhibitory neurons. These results underscore the inhibition of the ACC as a clinical alternative in inhibiting chronic pain, and leads to a better understanding of the pain processing circuitry of the cingulate cortex.
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影响因子：
13.9
作者：
Fenno L;Yizhar O;Deisseroth K
通讯作者：
Deisseroth K
DOI：
10.1126/science.1232437
发表时间：
2013-04-12
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Kim TI;McCall JG;Jung YH;Huang X;Siuda ER;Li Y;Song J;Song YM;Pao HA;Kim RH;Lu C;Lee SD;Song IS;Shin G;Al-Hasani R;Kim S;Tan MP;Huang Y;Omenetto FG;Rogers JA;Bruchas MR
通讯作者：
Bruchas MR
影响因子：
2.5
作者：
Koyama, T;Kato, K;Mikami, A
通讯作者：
Mikami, A
影响因子：
5.3
作者：
LaGraize, SC;Labuda, CJ;Fuchs, PN
通讯作者：
Fuchs, PN
影响因子：
64.8
作者：
COBB, SR;BUHL, EH;SOMOGYI, P
通讯作者：
SOMOGYI, P