Mutagenesis of RpoE-like sigma factor genes in Bdellovibrio reveals differential control of groEL and two groES genes.
Mutagenesis of RpoE-like sigma factor genes in Bdellovibrio reveals differential control of groEL and two groES genes.
复制标题
DOI：
10.1186/1471-2180-12-99
复制
发表时间：
2012-06-07
期刊：
影响因子：
4.2
通讯作者：
Sockett RE
中科院分区：
文献类型：
--
作者：
Lambert C;Till R;Hobley L;Sockett RE
细菌蛭弧菌HD100在进入、捕食和离开其他细菌或在没有猎物的情况下轴向生长时，必须对各种环境条件做出反应来调节基因。除了“看家”西格玛因子外，它的基因组还编码几种交替的西格玛因子，包括2个IV-RpoE类蛋白，这可能参与了对其捕食性生活方式的复杂调控。我们发现Bdellovibrio的一个sigma因子基因bd3314在捕食性或非捕食性生长状态下都不能被删除，因此可能是必需的，很可能是一个替代的sigma 70。两个IV类sigma因子基因中的一个bd0881的缺失会影响鞭毛基因的调控，导致捕食效率降低，尽管这不是由于运动性的改变；第二个基因bd0743的缺失表明它通常会抑制伴侣基因的表达，有趣的是，我们发现另一个groes基因在捕食周期的某个时间点表达，在捕食周期的某个时间点，在被捕食者溶解之前，将发生强烈的蛋白质合成。我们在了解交替西格玛因子如何调节蛭弧菌捕食生命周期的不同过程中迈出了第一步，并发现由其中一种因子调控的交替伴侣在生命周期的不同阶段表达。
Bdellovibrio bacteriovorus HD100 must regulate genes in response to a variety of environmental conditions as it enters, preys upon and leaves other bacteria, or grows axenically without prey. In addition to “housekeeping” sigma factors, its genome encodes several alternate sigma factors, including 2 Group IV-RpoE-like proteins, which may be involved in the complex regulation of its predatory lifestyle. We find that one sigma factor gene, bd3314, cannot be deleted from Bdellovibrio in either predatory or prey-independent growth states, and is therefore possibly essential, likely being an alternate sigma 70. Deletion of one of two Group IV-like sigma factor genes, bd0881, affects flagellar gene regulation and results in less efficient predation, although not due to motility changes; deletion of the second, bd0743, showed that it normally represses chaperone gene expression and intriguingly we find an alternative groES gene is expressed at timepoints in the predatory cycle where intensive protein synthesis at Bdellovibrio septation, prior to prey lysis, will be occurring. We have taken the first step in understanding how alternate sigma factors regulate different processes in the predatory lifecycle of Bdellovibrio and discovered that alternate chaperones regulated by one of them are expressed at different stages of the lifecycle.
登录
查看更多内容
DOI：
10.1007/bf00338097
发表时间：
1986-01-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
ROGERS, M;EKATERINAKI, N;SHERRATT, D
通讯作者：
SHERRATT, D
影响因子：
3.2
作者：
VARON, M;SHILO, M
通讯作者：
SHILO, M
影响因子：
3.2
作者：
Evans, Katy J.;Lambert, Carey;Sockett, R. Elizabeth
通讯作者：
Sockett, R. Elizabeth
DOI：
10.1099/00221287-40-3-317
发表时间：
1965-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
SHILO, M;BRUFF, B
通讯作者：
BRUFF, B
影响因子：
9.8
作者：
Lane, William J;Darst, Seth A
通讯作者：
Darst, Seth A