The impact of insecticide applications on the dynamics of resistance: The case of four Aedes aegypti populations from different Brazilian regions.
The impact of insecticide applications on the dynamics of resistance: The case of four Aedes aegypti populations from different Brazilian regions.
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DOI：
10.1371/journal.pntd.0006227
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发表时间：
2018-03
影响因子：
3.8
通讯作者：
Valle D
中科院分区：
文献类型：
--
作者：
Garcia GA;David MR;Martins AJ;Maciel-de-Freitas R;Linss JGB;Araújo SC;Lima JBP;Valle D
在热带地区，使用杀虫剂仍然是控制埃及伊蚊的重要策略，埃及伊蚊是登革热、基孔肯雅和寨卡病毒的主要媒介。然而，增加杀虫剂抗性在Ae。埃及人口可能会长期阻碍杀虫剂的效力。必须通过评估杀虫剂抗药性的发生频率和机制，了解抗药性的动态和演变。对4种巴西伊蚊的抗药性进行了研究。对埃及人口进行了监测。对该国使用的主要杀虫剂进行了定量生物测定：杀成虫剂溴氰菊酯（一种拟除虫菊酯-PY）和杀幼虫剂、灭幼磷（一种有机磷酸酯）和灭幼脲（一种几丁质合成抑制剂）。在所有种群中均检测到对替米磷的抗性，但随着时间的推移，抗性略有下降，这可能是由于田间使用的中断。所有病媒种群均对该国最近为控制Ae而引进的灭幼脲敏感。埃及人。3个种群对溴氰菊酯的抗性均极高。分子测定研究了电压门控钠通道（NaV）（PY靶位点）1011、1016和1534位的置换。鉴定了与高PY抗性水平相关的置换Val 1016 Ile和Phe 1534 Cys的频率升高。生化分析检测到两个解毒酶类代谢抗性，谷胱甘肽-S-转移酶和酯酶的活动的变化。在最近杀虫剂使用和每个地方登革热发病率记录的背景下对所获得的结果进行了评价。四个Ae。评估的埃及种群对神经毒性杀虫剂、替美磷和溴氰菊酯具有抗药性。然而，它们仍然对灭幼脲敏感。成虫对PY的抗性和国内杀虫剂的应用之间可能存在的相关性进行了讨论，指出需要采取提高认识的措施，正确利用公民。这项工作旨在为Ae的高效合理管理做出贡献。埃及伊蚊控制幼虫和成虫。在埃及伊蚊传播的病原体中，登革热病毒是最重要的，因为受影响或处于危险中的人数众多，并且全球死亡率很高。确认，Ae。埃及人也是寨卡病毒、基孔肯雅热和城市黄热病的病媒，对公共卫生造成严重后果，表明有必要重新评估目前的病媒控制战略。虽然人们日益认识到社会参与和社区参与对预防高水平虫害的重要性，但杀虫剂仍被视为重要的病媒控制工具。然而，大量和不加区别地采用杀虫剂来控制幼虫和成虫有助于抗药性的传播。特别是，国内使用的杀成虫剂，特别是在流行季节，被认为是诱导高水平的抗性在Ae。埃及人。然而，杀虫剂中断对田间种群抗药性的影响却研究得较少。我们评估，在四个巴西地区超过一年，登革热病媒种群的耐药性在该国使用的主要杀虫剂的动态。并对主要的抗性机制进行了探讨。考虑到登革热爆发、公共和私人化学品控制和杀虫剂抗药性之间的潜在关系，对数据进行了讨论。
In the tropics, the utilization of insecticides is still an important strategy for controlling Aedes aegypti, the principle vector of dengue, chikungunya and Zika viruses. However, increasing insecticide resistance in Ae. aegypti populations might hinder insecticide efficacy on a long-term basis. It will be important to understand the dynamics and evolution of insecticide resistance by assessing its frequency and the mechanisms by which it occurs. The insecticide resistance status of four Brazilian Ae. aegypti populations was monitored. Quantitative bioassays with the major insecticides employed in the country was performed: the adulticide deltamethrin (a pyrethroid—PY) and the larvicides, temephos (an organophosphate) and diflubenzuron (a chitin synthesis inhibitor). Temephos resistance was detected in all populations although exhibiting a slight decrease over time probably due to the interruption of field use. All vector populations were susceptible to diflubenzuron, recently introduced in the country to control Ae. aegypti. Resistance against deltamethrin was extremely high in three populations. Molecular assays investigated substitutions n the voltage gated sodium channel (NaV), the PY target site, at positions 1011, 1016 and 1534. Elevated frequencies of substitutions Val1016Ile and Phe1534Cys related to high PY resistance levels were identified. Biochemical assays detected alterations in the activities of two detoxifying enzyme classes related to metabolic resistance, glutathion-S-transferases and esterases. The results obtained were evaluated in the context of both recent insecticide use and the records of dengue incidence in each locality. The four Ae. aegypti populations evaluated were resistant to the neurotoxic insecticides, temephos and deltamethrin. However, they were still susceptible to diflubenzuron. A probable correlation between adult insect resistance to PY and the domestic application of insecticides is discussed, pointing to the need for awareness measures regarding the correct utilization by citizens. This work aims to contribute to the efficient and rational management of Ae. aegypti control of both larvae and adults. Among the pathogens transmitted by Aedes aegypti, dengue virus is the most important due to the number of people affected or at risk and the high rate of mortality worldwide. The confirmation that Ae. aegypti is also the vector of Zika, chikungunya and urban yellow fever poses serious consequences for public health, pointing to the need of reevaluating current vector control strategies. Although there is growing recognition of the importance of social participation and community engagement to prevent high levels of infestation, insecticides are considered important vector control tools. Nevertheless, the massive and indiscriminate adoption of insecticides to control larvae and adults contributes to resistance spread. In particular, the domestic use of adulticides, especially in epidemic seasons, is assumed to induce high levels of resistance in Ae. aegypti populations. However, the consequences of insecticide interruption upon the resistance of field populations has been less investigated. We evaluated, in four Brazilian regions over one year, the dynamics of dengue vector population resistance to the principal insecticides used in the country. The main resistance mechanisms were also investigated. Data are discussed taking into account the potential relationship among dengue outbreaks, public and private chemical control and insecticide resistance.
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影响因子：
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作者：
Fares RC;Souza KP;Añez G;Rios M
通讯作者：
Rios M
DOI：
10.1590/s0074-02762005000400016
发表时间：
2005-07-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Braga, Ima Aparecida;Mello, Cícero Brasileiro;Valle, Denise
通讯作者：
Valle, Denise
影响因子：
--
作者：
Bellinato DF;Viana-Medeiros PF;Araújo SC;Martins AJ;Lima JB;Valle D
通讯作者：
Valle D
DOI：
10.4269/ajtmh.2010.09-0346
发表时间：
2010-01-01
影响因子：
3.3
作者：
Luis San Martin, Jose;Brathwaite, Olivia;Guzman, Maria G.
通讯作者：
Guzman, Maria G.
DOI：
10.1590/s0074-02762005000400017
发表时间：
2005-07-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
da-Cunha, Marcella Pereira;Lima, José Bento Pereira;Valle, Denise
通讯作者：
Valle, Denise