Fusarium head blight resistance in European winter wheat: insights from genome-wide transcriptome analysis.
Fusarium head blight resistance in European winter wheat: insights from genome-wide transcriptome analysis.
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欧洲冬小麦赤霉病抗性：来自全基因组转录组分析的见解。
DOI：
10.1186/s12864-021-07800-1
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发表时间：
2021-06-24
期刊：
影响因子：
4.4
通讯作者：
Buerstmayr H
中科院分区：
文献类型：
--
作者：
Buerstmayr M;Wagner C;Nosenko T;Omony J;Steiner B;Nussbaumer T;Mayer KFX;Buerstmayr H
小麦赤霉病（Fusarium Head Blight，FHB）是一种世界性的小麦病害。赤霉病抗性是由多个中效QTL的联合效应控制的。开花性状，特别是花药挤出的程度，与赤霉病抗性密切相关。为了表征赤霉病抗性的遗传基础，我们生成并分析了96个欧洲冬小麦基因型对禾谷镰刀菌（Fg）感染的反应的表型和基因表达数据，其中包括几个含有来自高抗性亚洲品种Sumai 3的基因渗入的品系。这96个品系代表了广泛的FHB抗性，并根据其表型FHB严重程度评分分配到亚组。进行比较分析，以连接响应Fg感染的亚组特异性表达谱与FHB抗性水平。总的来说，超过12，300个小麦基因是镰刀菌反应的。Fg诱导的核心基因组在不同的抗性群体中是共同的，这表明基础防御反应机制的激活在很大程度上独立于小麦品系的抗性水平。在易感基因型中，纤维蛋白原诱导基因的表达水平更高。与更敏感的非Sumai 3系相比，Sumai 3衍生物表现出与细胞壁和植物型次生细胞壁生物发生相关的基因的更高的组成型表达，以及参与萜烯代谢的基因的更高的组成型和Fg诱导的表达。通过对FHB QTL Qfhs.ifa-5A的基因表达分析，确定了一个组成型表达的编码应激反应NST 1样蛋白的基因（TraesCS 5A 01 G211300 LC）为FHB抗性的候选基因。NST 1基因是花药药室内壁细胞次生壁生物合成的关键调控基因。胁迫反应NST 1类基因是否影响花药挤出，从而影响FHB抗性，有待进一步研究。诱导和预先存在的细胞壁成分和萜烯代谢产物有助于抵抗和限制真菌定植earlyon. In相反，过量的基因表达指导植物防御反应对程序性细胞死亡，有利于坏死营养生长的Fg病原体，从而可能导致增加真菌定植。在线版本包含补充材料，可通过10.1186/s12864-021-07800-1获得。
Fusarium head blight (FHB) is a devastating disease of wheat worldwide. Resistance to FHB is quantitatively controlled by the combined effects of many small to medium effect QTL. Flowering traits, especially the extent of extruded anthers, are strongly associated with FHB resistance. To characterize the genetic basis of FHB resistance, we generated and analyzed phenotypic and gene expression data on the response to Fusarium graminearum (Fg) infection in 96 European winter wheat genotypes, including several lines containing introgressions from the highly resistant Asian cultivar Sumai3. The 96 lines represented a broad range in FHB resistance and were assigned to sub-groups based on their phenotypic FHB severity score. Comparative analyses were conducted to connect sub-group-specific expression profiles in response to Fg infection with FHB resistance level. Collectively, over 12,300 wheat genes were Fusarium responsive. The core set of genes induced in response to Fg was common across different resistance groups, indicating that the activation of basal defense response mechanisms was largely independent of the resistance level of the wheat line. Fg-induced genes tended to have higher expression levels in more susceptible genotypes. Compared to the more susceptible non-Sumai3 lines, the Sumai3-derivatives demonstrated higher constitutive expression of genes associated with cell wall and plant-type secondary cell wall biogenesis and higher constitutive and Fg-induced expression of genes involved in terpene metabolism. Gene expression analysis of the FHB QTL Qfhs.ifa-5A identified a constitutively expressed gene encoding a stress response NST1-like protein (TraesCS5A01G211300LC) as a candidate gene for FHB resistance. NST1 genes are key regulators of secondary cell wall biosynthesis in anther endothecium cells. Whether the stress response NST1-like gene affects anther extrusion, thereby affecting FHB resistance, needs further investigation. Induced and preexisting cell wall components and terpene metabolites contribute to resistance and limit fungal colonization early on. In contrast, excessive gene expression directs plant defense response towards programmed cell death which favors necrotrophic growth of the Fg pathogen and could thus lead to increased fungal colonization. The online version contains supplementary material available at 10.1186/s12864-021-07800-1.
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影响因子：
4.6
作者：
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