THAP and ATF-2 regulated sterol carrier protein-2 promoter activities in the larval midgut of the yellow fever mosquito, Aedes aegypti.
THAP and ATF-2 regulated sterol carrier protein-2 promoter activities in the larval midgut of the yellow fever mosquito, Aedes aegypti.
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DOI：
10.1371/journal.pone.0046948
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Lan Q
中科院分区：
文献类型：
--
作者：
Peng R;Fu Q;Hong H;Schwaegler T;Lan Q
固醇载体蛋白-2（SCP-2）在埃及伊蚊的整个生命周期中的表达表现出独特的时间/空间模式。为了确定负责AeSCP-2转录的幼虫时间/空间调控的转录因子，AeSCP-2启动子活性通过瞬时转染启动子/报告基因测定在体内进行了研究。AeSCP-2转录起始位点上游-1.3 kb的调控序列被发现对体内时间/空间启动子活性至关重要。有趣的是，-1.6 kb的启动子序列有效地驱动了幼虫中肠特异性siRNA的表达，表明-1.6 kb的上游序列足以产生时空AeSCP-2转录活性。通过标记的-1.6/-1.3 kb DNA探针下拉和蛋白质组学分析，在摄食幼虫的中肠核提取物中鉴定出四种转录因子。共转染的启动子/报告基因与诱导siRNA表达的每个转录因子进行确认的调控功能的个别转录因子对AeSCP-2的转录活性在幼虫中肠。结果表明，两个已鉴定的转录因子，死亡相关蛋白（THAP）和转录激活因子-2（ATF-2），通过转录起始位点5′上游-1.6至-1.3 kb之间的调控序列，拮抗性地控制摄食幼虫中肠AeSCP-2的转录活性。THAP和ATF-2的体内表达敲低导致发育进程的显著变化，这可能部分是由于它们对AeSCP-2表达的影响。
Expression of sterol carrier protein-2 (SCP-2) in Aedes aegypti shows a distinct temporal/spatial pattern throughout the life cycle. In order to identify the transcription factors responsible for the larval temporal/spatial regulation of AeSCP-2 transcription, AeSCP-2 promoter activities were studied in vivo via transient transfection of promoter/reporter gene assays. Regulatory sequences upstream −1.3 kb of the transcription start site of AeSCP-2 were found to be critical for the in vivo temporal/spatial promoter activity. Interestingly, the −1.6 kb promoter sequence efficiently drove the larval midgut-specific siRNA expression, indicating that the −1.6 kb upstream sequence is sufficient for temporal/spatial AeSCP-2 transcriptional activity. Four transcription factors were identified in the midgut nuclear extract from feeding larvae via labeled −1.6/−1.3 kb DNA probe pull-down and proteomic analysis. Co-transfection of the promoter/reporter gene with inducible siRNA expression of each transcription factor was performed to confirm the regulatory function of individual transcription factor on AeSCP-2 transcriptional activities in the larval midgut. The results indicate that two of the identified transcription factors, Thanatos-associated protein (THAP) and activating transcription factor-2 (ATF-2), antagonistically control AeSCP-2 transcriptional activity in the midgut of feeding larvae via the regulatory sequences between −1.6 to −1.3 kb 5′ upstream of the transcription start site. In vivo expression knockdown of THAP and ATF-2 resulted in significant changes in developmental progression, which may be partially due to their effects on AeSCP-2 expression.
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影响因子：
2.6
作者：
Krebs, KC;Lan, Q
通讯作者：
Lan, Q
DOI：
10.1073/pnas.0406882102
发表时间：
2005-05-10
影响因子：
11.1
作者：
Clouaire, T;Roussigne, M;Girard, JP
通讯作者：
Girard, JP
DOI：
10.1016/j.bbrc.2007.03.194
发表时间：
2007-06-01
影响因子：
3.1
作者：
Lopez, Dayami;Niesen, Melissa;McLean, Mark P.
通讯作者：
McLean, Mark P.
影响因子：
9.2
作者：
Boxem, M;van den Heuvel, S
通讯作者：
van den Heuvel, S
影响因子：
3.3
作者：
Chesney, MA;Kidd, AR;Kimble, J
通讯作者：
Kimble, J