Biochemical analysis of spontaneous fepA mutants of Escherichia coli.
Biochemical analysis of spontaneous fepA mutants of Escherichia coli.
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大肠杆菌自发 fepA 突变体的生化分析。
DOI：
10.1099/00221287-134-5-1355
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Earhart,CF
中科院分区：
文献类型：
--
作者：
Elish,ME;Pierce,JR;Earhart,CF
大肠杆菌的fepA基因编码铁肠杆菌素的外膜受体蛋白。先前的遗传学研究表明，fep突变频繁发生，并表明大多数突变是缺失。在这项工作中，通过酶测定（肠杆菌素合酶和肠杆菌素酯酶）和 DNA 杂交研究分析了七个自发的 fepA 突变。在UT500和UT700这两个菌株中，突变仅限于fepA基因。其余突变体中，突变均为大缺失；在一些案例中，27 kb 或更多的 DNA 丢失了。这些缺失全部消除了肠杆菌素基因簇的大约左半部分，从fepC基因附近逆时针延伸到染色体中。识别出至少三个顺时针端点，并检测到至少两个逆时针端点。缺失之间终点的变化反对正常转座子参与其形成。此外，在肠杆菌素基因簇DNA与lacPOZ和pSC101之间发现了意想不到的同源性。
ThefepAgene ofEscherichia coliencodes the outer-membrane receptor protein for ferrienterobactin. Previous genetic studies indicated thatfepAmutations occur frequently and suggested that most of the mutations were deletions. In this work seven spontaneousfepAmutations were analysed by enzyme assay (enterobactin synthase and enterobactin esterase) and by DNA hybridization studies. In two strains, UT500 and UT700, the mutations were confined to thefepAgene. In the remaining mutants, the mutations were large deletions; in several cases, 27 kb or more of DNA had been lost. The deletions, all of which eliminated approximately the left half of the enterobactin gene cluster, extended from the vicinity of thefepCgene counterclockwise into the chromosome. A minimum of three clockwise endpoints were identified and at least two counterclockwise endpoints were detected. The variation in endpoints among the deletions argues against the involvement of a normal transposon in their formation. Also, unexpected homology was found between enterobactin gene cluster DNA andlacPOZand pSC101.
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影响因子：
3.1
作者：
LINDHOLM, L;HOLMGREN, J;LYCKE, N
通讯作者：
LYCKE, N
DOI：
10.1099/00221287-76-2-417
发表时间：
1973-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
LONNROTH, I;HOLMGREN, J
通讯作者：
HOLMGREN, J
影响因子：
3.1
作者：
E. Remmers;R. Colwell;R. Goldsby
通讯作者：
R. Goldsby
霍乱肠毒素家族的表位。
DOI：
10.1093/clinids/9.3.544
发表时间：
1987
期刊：
Reviews of infectious diseases
影响因子：
--
作者：
Finkelstein,RA;Burks,MF;Zupan,A;Dallas,WS;Jacob,CO;Ludwig,DS
通讯作者：
Ludwig,DS
产肠毒素大肠杆菌和霍乱弧菌O1致病决定因素的进化起源
影响因子：
3.2
作者：
Tatsuo;Yamamoto;Takashi Gojobori;Takeshi Yokota
通讯作者：
Takeshi Yokota