Genetic and molecular characterization of determinant of six-rowed spike of barley carrying vrs1.a4
Genetic and molecular characterization of determinant of six-rowed spike of barley carrying vrs1.a4
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携带 vrs1.a4 的大麦六棱穗决定子的遗传和分子特征
DOI：
10.1007/s00122-021-03887-y
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发表时间：
2021-06
影响因子：
5.4
通讯作者：
Deng Guang-Bing
中科院分区：
文献类型：
--
作者：
Wang Jin-Hui;Xu Zhen-Mei;Qiu Xue-Bing;Li Li-Lan;Yu Shui-Yang;Li Tao;Tang Yan-Yan;Pu Xi;Zhang Juan-Yu;Zhang Hai-Li;Liang Jun-Jun;Tang Ya-Wei;Li Wei;Long Hai;Deng Guang-Bing
vrs1转录物丰度降低在携带vrs1的大麦六棱穗中的决定性作用。对A4进行了遗传学证实，并对其潜在原因进行了初步分析。六棱穗1 （vrs1）是大麦六棱穗表型的主要决定因子。Vrs1的等位基因已被广泛研究。等位基因vrs1。六棱大麦中a4的独特之处在于它与Vrs1具有相同的编码序列。双棱大麦中的B4。vrs1中行类型的行列式。A4载体还没有实验鉴定。这里，我们确定了Vrs1。B4在双排加入和vrs1。遗传分析显示，在这些材料中，单个核基因导致了行型改变。物理定位在2H染色体上发现了一个0.08 cm （~ 554 kb）的目标区间，其中Vrs1是最有可能的候选基因。Vrs1在定位群体或不同Vrs1后代中的进一步表达分析。B4和vrs1。A4细胞系证实vrs1表达下调。A4引起六划尖峰。调控序列分析发现vrs1中存在一个“TA”二核苷酸缺失。在编码区上游约1kb的“TA”串联重复序列富集区内的a4个载波。DNA甲基化水平不对应于表达差异，因此不影响Vrs1的表达。需要更多的证据来验证“TA”缺失与Vrs1表达下调之间的因果关系，从而导致六排尖峰表型。
Decisive role of reduced vrs1 transcript abundance in six-rowed spike of barley carrying vrs1.a4 was genetically proved and its potential causes were preliminarily analyzed. Six-rowed spike 1 (vrs1) is the major determinant of the six-rowed spike phenotype of barley (Hordeum vulgare L.). Alleles of Vrs1 have been extensively investigated. Allele vrs1.a4 in six-rowed barley is unique in that it has the same coding sequence as Vrs1.b4 in two-rowed barley. The determinant of row-type in vrs1.a4 carriers has not been experimentally identified. Here, we identified Vrs1.b4 in two-rowed accessions and vrs1.a4 in six-rowed accessions from the Qinghai–Tibet Plateau at high frequency. Genetic analyses revealed a single nuclear gene accounting for row-type alteration in these accessions. Physical mapping identified a 0.08-cM (~ 554-kb) target interval on chromosome 2H, wherein Vrs1 was the most likely candidate gene. Further analysis of Vrs1 expression in offspring of the mapping populations or different Vrs1.b4 and vrs1.a4 lines confirmed that downregulated expression of vrs1.a4 causes six-rowed spike. Regulatory sequence analysis found a single ‘TA’ dinucleotide deletion in vrs1.a4 carriers within a ‘TA’ tandem-repeat–enriched region ~ 1 kb upstream of the coding region. DNA methylation levels did not correspond to the expression difference and therefore did not affect Vrs1 expression. More evidence is needed to verify the causal link between the ‘TA’ deletion and the downregulated Vrs1 expression and hence the six-rowed spike phenotype.
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影响因子：
4.2
作者：
Pourkheirandish M;Komatsuda T
通讯作者：
Komatsuda T
影响因子：
12.3
作者：
Niederhuth CE;Bewick AJ;Ji L;Alabady MS;Kim KD;Li Q;Rohr NA;Rambani A;Burke JM;Udall JA;Egesi C;Schmutz J;Grimwood J;Jackson SA;Springer NM;Schmitz RJ
通讯作者：
Schmitz RJ
影响因子：
16.6
作者：
Bull H;Casao MC;Zwirek M;Flavell AJ;Thomas WTB;Guo W;Zhang R;Rapazote-Flores P;Kyriakidis S;Russell J;Druka A;McKim SM;Waugh R
通讯作者：
Waugh R
影响因子：
9.5
作者：
Thorvaldsdóttir H;Robinson JT;Mesirov JP
通讯作者：
Mesirov JP
影响因子：
3.7
作者：
Sawaya S;Bagshaw A;Buschiazzo E;Kumar P;Chowdhury S;Black MA;Gemmell N
通讯作者：
Gemmell N