Keeping It Together: Structures, Functions, and Applications of Viral Decoration Proteins.
Keeping It Together: Structures, Functions, and Applications of Viral Decoration Proteins.
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保持它在一起：病毒装饰蛋白的结构、功能和应用。
DOI：
10.3390/v12101163
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发表时间：
2020-10-14
期刊：
影响因子：
--
通讯作者：
Alexandrescu AT
中科院分区：
文献类型：
--
作者：
Dedeo CL;Teschke CM;Alexandrescu AT
装饰蛋白是病毒的辅助基因产物，装饰在一些病毒衣壳和病毒衣壳的表面，特别是有尾的dsDNA衣壳。这些蛋白质通常发挥“粘合”作用，加强衣壳对积累的内部压力，由于基因组包装，或环境的侮辱，如极端的温度或pH值。许多装饰蛋白提供替代功能，包括靶细胞识别，参与病毒组装，衣壳大小的决定，或调节宿主基因表达。目前具有高分辨率结构特征的例子分为五个主要折叠基序：β-郁金香，β-蝌蚪，OB-折叠，Ig-样和罕见的打结α-螺旋折叠。这些折叠基序中的大多数在病毒和靶细胞蛋白中具有结构同源物，表明水平基因转移在它们的进化中是重要的。装饰蛋白的寡聚化状态从单体到三聚体，其中后者最典型。装饰蛋白结合到衣壳上的各种位点，包括二十面体2-、3-和5-重对称轴，以及假对称位点。这些结合位点通常对应于衣壳晶格上的“弱点”。由于其独特的非共价结合病毒表面的能力，装饰蛋白越来越多地用于技术，其用途包括噬菌体展示，病毒功能化，疫苗接种和改进的纳米颗粒设计用于成像和药物递送。这些应用无疑将受益于我们对这些多功能病毒功能增强剂的进一步理解。
Decoration proteins are viral accessory gene products that adorn the surfaces of some phages and viral capsids, particularly tailed dsDNA phages. These proteins often play a “cementing” role, reinforcing capsids against accumulating internal pressure due to genome packaging, or environmental insults such as extremes of temperature or pH. Many decoration proteins serve alternative functions, including target cell recognition, participation in viral assembly, capsid size determination, or modulation of host gene expression. Examples that currently have structures characterized to high-resolution fall into five main folding motifs: β-tulip, β-tadpole, OB-fold, Ig-like, and a rare knotted α-helical fold. Most of these folding motifs have structure homologs in virus and target cell proteins, suggesting horizontal gene transfer was important in their evolution. Oligomerization states of decoration proteins range from monomers to trimers, with the latter most typical. Decoration proteins bind to a variety of loci on capsids that include icosahedral 2-, 3-, and 5-fold symmetry axes, as well as pseudo-symmetry sites. These binding sites often correspond to “weak points” on the capsid lattice. Because of their unique abilities to bind virus surfaces noncovalently, decoration proteins are increasingly exploited for technology, with uses including phage display, viral functionalization, vaccination, and improved nanoparticle design for imaging and drug delivery. These applications will undoubtedly benefit from further advances in our understanding of these versatile augmenters of viral functions.
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DOI：
10.1007/978-1-62703-751-8_16
发表时间：
2014-01-01
期刊：
VIRUS HYBRIDS AS NANOMATERIALS: METHODS AND PROTOCOLS
影响因子：
--
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
21.8
作者：
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DOI：
10.1073/pnas.1508355112
发表时间：
2015-11-03
影响因子：
11.1
作者：
Barr, Jeremy J.;Auro, Rita;Rohwer, Forest
通讯作者：
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DOI：
10.1073/pnas.1305923110
发表时间：
2013-06-25
影响因子：
11.1
作者：
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通讯作者：
Rohwer, Forest