Target search by an imported conjugative DNA element for a unique integration site along a bacterial chromosome during horizontal gene transfer.
Target search by an imported conjugative DNA element for a unique integration site along a bacterial chromosome during horizontal gene transfer.
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DOI：
10.1093/nar/gkad068
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发表时间：
2023-04-24
影响因子：
14.9
通讯作者：
中科院分区：
文献类型：
--
作者：
整合和共轭元件（ICEs）是可移动的遗传元件，可以通过接合转移到受体细胞。一些ice整合到宿主基因组的一个独特位点上。我们定量研究了ICE在枯草芽孢杆菌染色体中寻找其独特整合位点的过程。我们在单个细胞内实时跟踪ICEBs1和染色体整合位点的运动。ICEBs1表现出广泛的动力学行为，从快速的亚扩散位移在电池中纵横交错，到动力学捕获状态。染色体整合位点的移动是次扩散的，并且在纵向和横向运动之间表现出明显的动力学不对称，这突出了染色体结构和细菌内部异质性在搜索中的作用。成功的搜索和随后的重组进入整合位点是获得整合移动遗传元件的关键步骤。我们的发现为涉及大DNA分子的细胞内运输过程提供了新的见解。
Integrative and conjugative elements (ICEs) are mobile genetic elements that can transfer by conjugation to recipient cells. Some ICEs integrate into a unique site in the genome of their hosts. We studied quantitatively the process by which an ICE searches for its unique integration site in the Bacillus subtilis chromosome. We followed the motion of both ICEBs1 and the chromosomal integration site in real time within individual cells. ICEBs1 exhibited a wide spectrum of dynamical behaviors, ranging from rapid sub-diffusive displacements crisscrossing the cell, to kinetically trapped states. The chromosomal integration site moved sub-diffusively and exhibited pronounced dynamical asymmetry between longitudinal and transversal motions, highlighting the role of chromosomal structure and the heterogeneity of the bacterial interior in the search. The successful search for and subsequent recombination into the integration site is a key step in the acquisition of integrating mobile genetic elements. Our findings provide new insights into intracellular transport processes involving large DNA molecules.
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影响因子：
2.7
作者：
Alpern, S
通讯作者：
Alpern, S
DOI：
10.1103/physreve.91.062716
发表时间：
2015-06
期刊：
Physical review. E, Statistical, nonlinear, and soft matter physics
影响因子：
--
作者：
Backlund MP;Joyner R;Moerner WE
通讯作者：
Moerner WE
影响因子：
4.5
作者：
Guglielmini J;Quintais L;Garcillán-Barcia MP;de la Cruz F;Rocha EP
通讯作者：
Rocha EP
DOI：
10.1146/annurev-cellbio-100814-125211
发表时间：
2015
影响因子：
11.3
作者：
Badrinarayanan A;Le TB;Laub MT
通讯作者：
Laub MT
影响因子：
3.6
作者：
Cabrera, JE;Jin, DJ
通讯作者：
Jin, DJ