Phosphonate production by marine microbes: Exploring new sources and potential function.
Phosphonate production by marine microbes: Exploring new sources and potential function.
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DOI：
10.1073/pnas.2113386119
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发表时间：
2022-03-15
影响因子：
11.1
通讯作者：
Repeta DJ
中科院分区：
文献类型：
--
作者：
Acker M;Hogle SL;Berube PM;Hackl T;Coe A;Stepanauskas R;Chisholm SW;Repeta DJ
膦酸盐是一类以高度稳定的C-P键为特征的磷代谢物。膦酸盐在海水中积累到高浓度，为大部分海洋甲烷生产提供燃料，并为栖息在贫营养海洋营养有限区域的微生物提供磷源。在这里，我们表明，15%的所有浮游细菌在海洋表面的基因膦酸盐合成，大多数属于丰富的组原绿球藻和SAR 11。基因组和化学证据表明，膦酸盐被纳入细胞表面的磷酸糖蛋白，可能会采取行动，以减轻细胞死亡率放牧和病毒裂解。这些结果强调了在数量丰富的海洋细菌群体中相对罕见但高度表达的特征的巨大全球生物地球化学影响。膦酸酯是具有特征性C-P键的有机磷代谢物。它们在海洋环境中无处不在，它们的退化广泛支持生态系统生产力，它们是海洋磷循环的关键组成部分。然而，微生物生产者，维持大量的海洋库存的膦酸盐以及生理和生态作用的膦酸盐是谜。在这里，我们表明，膦酸盐合成基因是罕见的，但广泛分布在不同的细菌和古细菌，包括原绿球藻和SAR 11，在海洋中的两个主要群体的细菌。此外，我们表明，原绿球藻可以分配超过40%的总细胞磷配额对膦酸盐生产。然而，我们没有发现任何证据表明原绿球藻使用磷酸盐的盈余P存储，几乎所有的生产者基因组缺乏必要的基因降解和同化膦酸盐。相反，我们假设膦酸盐与细胞表面糖蛋白相关，表明膦酸盐介导细胞与周围环境之间的生态相互作用。我们的研究结果表明，贫营养的表面海洋膦酸盐池是由一个相对较小的比例的浮游细菌细胞分配的一个显着的一部分，他们的P配额向次生代谢和远离生长和繁殖。
Phosphonates are a class of phosphorus metabolites characterized by a highly stable C-P bond. Phosphonates accumulate to high concentrations in seawater, fuel a large fraction of marine methane production, and serve as a source of phosphorus to microbes inhabiting nutrient-limited regions of the oligotrophic ocean. Here, we show that 15% of all bacterioplankton in the surface ocean have genes phosphonate synthesis and that most belong to the abundant groups Prochlorococcus and SAR11. Genomic and chemical evidence suggests that phosphonates are incorporated into cell-surface phosphonoglycoproteins that may act to mitigate cell mortality by grazing and viral lysis. These results underscore the large global biogeochemical impact of relatively rare but highly expressed traits in numerically abundant groups of marine bacteria. Phosphonates are organophosphorus metabolites with a characteristic C-P bond. They are ubiquitous in the marine environment, their degradation broadly supports ecosystem productivity, and they are key components of the marine phosphorus (P) cycle. However, the microbial producers that sustain the large oceanic inventory of phosphonates as well as the physiological and ecological roles of phosphonates are enigmatic. Here, we show that phosphonate synthesis genes are rare but widely distributed among diverse bacteria and archaea, including Prochlorococcus and SAR11, the two major groups of bacteria in the ocean. In addition, we show that Prochlorococcus can allocate over 40% of its total cellular P-quota toward phosphonate production. However, we find no evidence that Prochlorococcus uses phosphonates for surplus P storage, and nearly all producer genomes lack the genes necessary to degrade and assimilate phosphonates. Instead, we postulate that phosphonates are associated with cell-surface glycoproteins, suggesting that phosphonates mediate ecological interactions between the cell and its surrounding environment. Our findings indicate that the oligotrophic surface ocean phosphonate pool is sustained by a relatively small fraction of the bacterioplankton cells allocating a significant portion of their P quotas toward secondary metabolism and away from growth and reproduction.
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影响因子：
3.7
作者：
Dyhrman ST;Jenkins BD;Rynearson TA;Saito MA;Mercier ML;Alexander H;Whitney LP;Drzewianowski A;Bulygin VV;Bertrand EM;Wu Z;Benitez-Nelson C;Heithoff A
通讯作者：
Heithoff A
影响因子：
2.5
作者：
Christaki, U;Van Wambeke, F;Dolan, JR
通讯作者：
Dolan, JR
DOI：
10.1007/978-3-319-24945-2_8
发表时间：
2016-01-01
期刊：
PHYSIOLOGY OF MICROALGAE
影响因子：
--
作者：
Dyhrman, Sonya T.
通讯作者：
Dyhrman, Sonya T.
DOI：
10.1093/bioinformatics/btz848
发表时间：
2019-11-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Chaumeil PA;Mussig AJ;Hugenholtz P;Parks DH
通讯作者：
Parks DH
影响因子：
48
作者：
Buchfink, Benjamin;Xie, Chao;Huson, Daniel H.
通讯作者：
Huson, Daniel H.