Environmental surveillance identifies multiple introductions of MRSA CC398 in an Equine Veterinary Hospital in the UK, 2011-2016.

Environmental surveillance identifies multiple introductions of MRSA CC398 in an Equine Veterinary Hospital in the UK, 2011-2016.
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DOI:
10.1038/s41598-017-05559-8
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发表时间:
2017-07-14
期刊:
影响因子:
4.6
通讯作者:
Timofte D
Timofte D
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Bortolami A;Williams NJ;McGowan CM;Kelly PG;Archer DC;Corrò M;Pinchbeck G;Saunders CJ;Timofte D

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2011 年至 2016 年,英国一家马转诊兽医医院实施了细菌环境和手术部位感染 (SSI) 监测,并鉴定出 81 株耐甲氧西林金黄色葡萄球菌 (MRSA) 分离株。 2016 年初发生了一批 MRSA SSI,经多位点序列分型 (MLST) 确认分离株为 ST398,这促使人们对过去五年从环境 (n = 62)、SSI (n = 6) 和手板 (n = 6) 中获得的所有 MRSA 分离株进行回顾性分析。其中 65 个分离株进行了 CC398 分型,并进一步对其中的选择 (n = 38) 进行了抗性和毒力基因、SCCmec 和 spa 分型的表征。总体而言,62/540 (11.5%) 的环境样本、6/81 的手板样本 (7.4%) 和 13/208 的 SSI (6.3%) 中均发现了 MRSA。 spa t011 是最常见的 (24/38),基于重复模式 (BURP) 分析将 spa t011 确定为五年来确定的主要 spa CC (spa CC011/3423) 的两个小组创始人之一。然而,还发现了 3 个单身个体(t073、t786、t064),表明它们被单独引入医院环境。这项长期 MRSA 监测研究揭示了英国一家马科医院多次引入 MRSA CC398,确定了一种新出现的人畜共患病原体,迄今为止在英国仅零星记录。
Bacterial environmental and surgical site infection (SSI) surveillance was implemented from 2011–2016 in a UK Equine Referral Veterinary Hospital and identified 81 methicillin-resistant Staphylococcus aureus (MRSA) isolates. A cluster of MRSA SSIs occurred in early 2016 with the isolates confirmed as ST398 by multilocus sequence typing (MLST), which prompted retrospective analysis of all MRSA isolates obtained from the environment (n = 62), SSIs (n = 13) and hand plates (n = 6) in the past five years. Sixty five of these isolates were typed to CC398 and a selection of these (n = 38) were further characterised for resistance and virulence genes, SCCmec and spa typing. Overall, MRSA was identified in 62/540 (11.5%) of environmental samples, 6/81 of the hand-plates (7.4%) and 13/208 of the SSIs (6.3%). spa t011 was the most frequent (24/38) and Based Upon Repeat Pattern (BURP) analysis identified spa t011 as one of the two group founders of the main spa CC identified across the five years (spa CC011/3423). However, 3 singletons (t073, t786, t064) were also identified suggesting separate introductions into the hospital environment. This long-term MRSA surveillance study revealed multiple introductions of MRSA CC398 in a UK Equine Hospital, identifying an emerging zoonotic pathogen so far only sporadically recorded in the UK.
耐甲氧西林金黄色葡萄球菌的大流行,流行病和零星克隆的现场指南。
DOI: 10.1371/journal.pone.0017936
发表时间: 2011-04-06
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发表时间: 2011-06-01
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在1999年至2011年,丹麦无牲畜接触的患者中,抗甲基肽的金黄色葡萄球菌CC398是疾病的越来越多的原因。
DOI: 10.2807/1560-7917.es.2015.20.37.30021
发表时间: 2015
期刊: Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin
影响因子: --
作者:
Larsen J;Petersen A;Sørum M;Stegger M;van Alphen L;Valentiner-Branth P;Knudsen LK;Larsen LS;Feingold B;Price LB;Andersen PS;Larsen AR;Skov RL
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DOI: 10.1016/s0378-1135(01)00396-0
发表时间: 2001-10-01
影响因子: 3.3
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