Livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) as causes of human infection and colonization in Germany.
Livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) as causes of human infection and colonization in Germany.
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DOI：
10.1371/journal.pone.0055040
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Friedrich AW
中科院分区：
文献类型：
--
作者：
Köck R;Schaumburg F;Mellmann A;Köksal M;Jurke A;Becker K;Friedrich AW
猪、牛和家禽都有MRSA的定植，这种MRSA通过动物直接接触、环境污染或肉类向人类的人畜共患传播是一个值得关注的问题。家畜相关（LA） MRSA多属于克隆复合体（CC） 398，由多位点序列分型确定。然而，在德国的家畜中也检测到其他克隆谱系的MRSA，包括CC5、CC9和CC97。在荷兰-德国网络项目（EUREGIO）的框架内，从临床和筛选标本（2008年1月至2012年6月）中分离出14,036例MRSA，这些标本来自医院的人类患者以及德国一个以高密度畜牧生产为特征的地区的一般或专业实践，对金黄色葡萄球菌蛋白a （spa）序列进行分型。通过分析已在德国牲畜中报道的家畜指示剂（LI） spa型的检测结果，确定了人MRSA分离株中假定的LA-MRSA的流行程度。在MRSA分离株中共检出578种spa类型。LI spa型t011、t034、t108、t1451、t2011、t571、t1456、t1250、t1255、t1580、t2970、t2346、t1344、t2576、t2330和t2510（均为LA-MRSA CC398的指示型）占所有人分离株的18.6%。LI spa型t1430 （CC9）、t3992 （CC97）、t002 （CC5）和t007 （CC30）分别占所有人MRSA分离株的0.14%、0.01%、1.01%和0.04%。与CC398相关的LI spa类型占筛选样本中所有MRSA的23%，临床标本中分离物的比例各不相同，脑脊液中为0%，血培养中为8%，深呼吸道液中为14%。我们的研究结果表明，LA-MRSA是人类感染的主要原因，并强调需要密切监测。虽然LA-MRSA CC398占主导地位，但应该监测其他克隆谱系中推定的LA-MRSA的发生。
Pigs, cattle and poultry are colonized with MRSA and the zoonotic transmission of such MRSA to humans via direct animal contact, environmental contaminations or meat are a matter of concern. Livestock-associated (LA) MRSA are mostly belonging to clonal complex (CC) 398 as defined by multilocus sequence typing. However, MRSA of other clonal lineages including CC5, CC9 and CC97 have also been detected in livestock animals in Germany. Within the framework of a Dutch-German network project (EUREGIO), 14,036 MRSA isolated from clinical and screening specimens (January 2008 - June 2012) derived from human patients in hospitals as well as general or specialized practices in a German region characterized by a high density of livestock production, were subjected to S. aureus protein A (spa) sequence typing. The prevalence of putative LA-MRSA among the human MRSA isolates was determined by analyzing the detection of livestock-indicator (LI) spa types which had already been reported in German livestock. Overall, 578 spa types were detected among the MRSA isolates. LI spa types t011, t034, t108, t1451, t2011, t571, t1456, t1250, t1255, t1580, t2970, t2346, t1344, t2576, t2330 and t2510 (all of which are indicative for LA-MRSA CC398) accounted for 18.6% of all human isolates. The LI spa types t1430 (CC9), t3992 (CC97), t002 (CC5) and t007 (CC30) were found in 0.14%, 0.01%, 1.01% and 0.04% of all human MRSA isolates, respectively. LI spa types associated with CC398 represented 23% of all MRSA from screening samples and a varying proportion among isolates from clinical specimens ranging between 0% in cerebrospinal fluid, 8% in blood cultures and 14% in deep respiratory fluids. Our findings indicate that LA-MRSA are a major cause for human infection and stress the need for close surveillance. Although LA-MRSA CC398 predominates, the occurrence of putative LA-MRSA from other clonal lineages should be monitored.
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影响因子：
6.4
作者：
Price LB;Stegger M;Hasman H;Aziz M;Larsen J;Andersen PS;Pearson T;Waters AE;Foster JT;Schupp J;Gillece J;Driebe E;Liu CM;Springer B;Zdovc I;Battisti A;Franco A;Zmudzki J;Schwarz S;Butaye P;Jouy E;Pomba C;Porrero MC;Ruimy R;Smith TC;Robinson DA;Weese JS;Arriola CS;Yu F;Laurent F;Keim P;Skov R;Aarestrup FM
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影响因子：
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通讯作者：
Witte W