A field guide to pandemic, epidemic and sporadic clones of methicillin-resistant Staphylococcus aureus.
A field guide to pandemic, epidemic and sporadic clones of methicillin-resistant Staphylococcus aureus.
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耐甲氧西林金黄色葡萄球菌的大流行，流行病和零星克隆的现场指南。
DOI：
10.1371/journal.pone.0017936
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发表时间：
2011-04-06
期刊：
影响因子：
3.7
通讯作者：
Ehricht R
中科院分区：
文献类型：
--
作者：
Monecke S;Coombs G;Shore AC;Coleman DC;Akpaka P;Borg M;Chow H;Ip M;Jatzwauk L;Jonas D;Kadlec K;Kearns A;Laurent F;O'Brien FG;Pearson J;Ruppelt A;Schwarz S;Scicluna E;Slickers P;Tan HL;Weber S;Ehricht R
近年来，耐甲氧西林金黄色葡萄球菌（MRSA）已成为一个真正的全球性挑战。除了众所周知的与医疗保健相关的克隆之外，新的菌株也出现在医院环境之外，在社区以及牲畜中。表达Panton-Valentine杀白细胞素（PVL）的毒性克隆的出现和传播是另一个令人担忧的原因。为了提供大流行、流行和散发菌株的概述，主要来自德国、英国、爱尔兰、法国、马耳他、阿布扎比、香港、澳大利亚、特立尼达和托巴哥的3,000多个MRSA临床和兽医分离株以及来自美国的一些参考菌株已经通过DNA微阵列分析进行了基因分型。该技术允许将MRSA分离株分配到34个不同的谱系，这些谱系可以基于非移动基因明确定义。结果与多位点序列分型数据一致。基于与这些谱系的隶属关系、SCCmec类型和PVL的存在或不存在来区分超过100种不同的菌株。这些菌株在这里描述主要是关于临床相关的抗生素耐药性和毒力相关的标志物，但也与流行病学和地理分布。研究结果表明，MRSA之间的生物多样性水平很高，特别是在含有SCCmec IV和V元素的菌株中。这些数据还表明MRSA中涉及SCC元件、噬菌体或其他移动的遗传元件和大规模染色体置换的基因重组率很高。
In recent years, methicillin-resistant Staphylococcus aureus (MRSA) have become a truly global challenge. In addition to the long-known healthcare-associated clones, novel strains have also emerged outside of the hospital settings, in the community as well as in livestock. The emergence and spread of virulent clones expressing Panton-Valentine leukocidin (PVL) is an additional cause for concern. In order to provide an overview of pandemic, epidemic and sporadic strains, more than 3,000 clinical and veterinary isolates of MRSA mainly from Germany, the United Kingdom, Ireland, France, Malta, Abu Dhabi, Hong Kong, Australia, Trinidad & Tobago as well as some reference strains from the United States have been genotyped by DNA microarray analysis. This technique allowed the assignment of the MRSA isolates to 34 distinct lineages which can be clearly defined based on non-mobile genes. The results were in accordance with data from multilocus sequence typing. More than 100 different strains were distinguished based on affiliation to these lineages, SCCmec type and the presence or absence of PVL. These strains are described here mainly with regard to clinically relevant antimicrobial resistance- and virulence-associated markers, but also in relation to epidemiology and geographic distribution. The findings of the study show a high level of biodiversity among MRSA, especially among strains harbouring SCCmec IV and V elements. The data also indicate a high rate of genetic recombination in MRSA involving SCC elements, bacteriophages or other mobile genetic elements and large-scale chromosomal replacements.
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影响因子：
14.2
作者：
Baranovich, T.;Zaraket, H.;Suzuki, H.
通讯作者：
Suzuki, H.
影响因子：
9.4
作者：
Adler, Amos;Givon-Lavi, Noga;Dagan, Ron
通讯作者：
Dagan, Ron
影响因子：
5.7
作者：
Campanile, Floriana;Bongiorno, Dafne;Stefani, Stefania
通讯作者：
Stefani, Stefania
影响因子：
3.7
作者：
Chheng K;Tarquinio S;Wuthiekanun V;Sin L;Thaipadungpanit J;Amornchai P;Chanpheaktra N;Tumapa S;Putchhat H;Day NP;Peacock SJ
通讯作者：
Peacock SJ
影响因子：
9.4
作者：
Aires-de-Sousa, Marta;Correia, Bruno;de Lencastre, Herminia
通讯作者：
de Lencastre, Herminia