In Vitro Antibacterial Activity of Unconjugated and Conjugated Bile Salts on Staphylococcus aureus.
In Vitro Antibacterial Activity of Unconjugated and Conjugated Bile Salts on Staphylococcus aureus.
复制标题
金黄色葡萄球菌上未缀合和共轭胆汁盐的体外抗菌活性。
DOI：
10.3389/fmicb.2017.01581
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发表时间：
2017
影响因子：
5.2
通讯作者：
Clarke SR
中科院分区：
文献类型：
--
作者：
Sannasiddappa TH;Lund PA;Clarke SR
胆盐是有效的抗微生物剂，是肠道先天防御的重要组成部分，可以保护肠道免受入侵生物的侵害。它们在确定肠道的微生物生态学方面起着重要作用，其水平的改变可导致病原体定植增加。我们先前已经证明了机会致病菌金黄色葡萄球菌在人类结肠模型中的存活。从而研究了S.金黄色葡萄球菌和胆盐是了解其在宿主肠道中定殖能力的重要因素。利用胆汁盐也可能为开发控制耐药细菌的治疗策略提供新的途径。尽管如此，胆汁盐对S.对金黄色葡萄球菌知之甚少。本实验研究了主要的非结合型和结合型胆盐对S。金黄色。未结合的胆汁酸盐在其最低抑菌浓度（胆酸和脱氧胆酸分别为20和1 mM）时可杀死S。金黄色葡萄球菌，并且这与增加的膜破裂和细胞内容物的渗漏有关。未结合的胆汁盐（分别为8和0.4 mM的胆酸和脱氧胆酸）和结合的胆汁盐（20 mM的甘氨胆酸和牛磺胆酸）在其亚抑制浓度下仍然能够通过破坏质子动力和增加膜渗透性来抑制生长。我们还证明了非结合胆汁盐对S.金黄色葡萄球菌比结合胆汁盐。
Bile salts are potent antimicrobial agents and are an important component of innate defenses in the intestine, giving protection against invasive organisms. They play an important role in determining microbial ecology of the intestine and alterations in their levels can lead to increased colonization by pathogens. We have previously demonstrated survival of the opportunistic pathogen Staphylococcus aureus in the human colonic model. Thus investigating the interaction between S. aureus and bile salts is an important factor in understanding its ability to colonize in the host intestine. Harnessing bile salts may also give a new avenue to explore in the development of therapeutic strategies to control drug resistant bacteria. Despite this importance, the antibacterial activity of bile salts on S. aureus is poorly understood. In this study, we investigated the antibacterial effects of the major unconjugated and conjugated bile salts on S. aureus. Several concentration-dependent antibacterial mechanisms were found. Unconjugated bile salts at their minimum inhibitory concentration (cholic and deoxycholic acid at 20 and 1 mM, respectively) killed S. aureus, and this was associated with increased membrane disruption and leakage of cellular contents. Unconjugated bile salts (cholic and deoxycholic acid at 8 and 0.4 mM, respectively) and conjugated bile salts (glycocholic and taurocholic acid at 20 mM) at their sub inhibitory concentrations were still able to inhibit growth through disruption of the proton motive force and increased membrane permeability. We also demonstrated that unconjugated bile salts possess more potent antibacterial action on S. aureus than conjugated bile salts.
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影响因子：
9.8
作者：
Bauer, TM;Steinbrückner, B;Blum, HE
通讯作者：
Blum, HE
影响因子：
1.7
作者：
CANTAFORA, A;DIBIASE, A;ALVARO, D
通讯作者：
ALVARO, D
影响因子：
13.5
作者：
Chang, CS;Chen, GH;Yeh, HZ
通讯作者：
Yeh, HZ
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4.9
作者：
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通讯作者：
Denyer, Stephen P.
影响因子：
3.4
作者：
Cheng, John T. J.;Hale, John D.;Straus, Suzana K.
通讯作者：
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