Air-assisted intranasal instillation enhances adenoviral delivery to the olfactory epithelium and respiratory tract.
Air-assisted intranasal instillation enhances adenoviral delivery to the olfactory epithelium and respiratory tract.
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作者：
鼻腔滴注用于将腺病毒载体输送到嗅觉上皮和呼吸道。然而，这种方法的成功受到了上皮和肺中不一致的传染性的影响。上皮细胞的感染可能部分受阻于腔的曲折结构、粘液的存在或后腔内气流不畅。腺病毒在不同的肺叶中的传递可能是不均匀的，远端细支气管会受到严重感染。目前规避这些问题的方法主要依赖于插管或气管内滴注。在这里，我们描述了一种无需手术干预即可显著提高腺病毒感染率的技术。我们使用压缩空气来增加滴入腺病毒的循环，导致上皮和肺部的感染加剧。这一过程简单明了，不需要专门的设备。在上皮中，神经元和支持细胞都被标记。在肺部，所有的肺叶都可以被感染，并穿透到最远端的细支气管处。压缩空气的使用也可能有助于增强其他所需的药物在上皮、中枢神经系统和呼吸道内的分布。
Intranasal instillation is used to deliver adenoviral vectors to the olfactory epithelium and respiratory tract. The success of this approach, however, has been tempered by inconsistent infectivity in both the epithelium and lungs. Infection of the epithelium may be hampered in part by the convoluted structure of the cavity, the presence of mucus, or poor airflow in the posterior cavity. Delivery of adenovirus to the lungs can be uneven in the various lobes, and distal bronchioles may be poorly infected. Current approaches to circumvent these issues rely principally on intubation or intratracheal instillation. Here we describe a technique that significantly improves adenoviral infectivity rates without requiring surgical intervention. We use compressed air to increase circulation of instilled adenovirus, resulting in enhanced infection in both the epithelium and lungs. This procedure is straightforward, simple to perform, and requires no specialized equipment. In the epithelium, neurons and sustentacular cells are both labeled. In the lungs, all lobes can be infected, with penetration to the most distal bronchioles. The use of compressed air will likely also be useful for enhancing the distribution of other, desired agents within the epithelium, CNS, and respiratory tract.
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影响因子：
3.8
作者：
Dhuria, Shyeilla V.;Hanson, Leah R.;Frey, William H., II
通讯作者：
Frey, William H., II
影响因子：
12.4
作者：
Bridle, Byram W.;Boudreau, Jeanette E.;Wan, Yonghong
通讯作者：
Wan, Yonghong
影响因子：
6.6
作者：
Danielyan, Lusine;Schaefer, Richard;Frey, William H., II
通讯作者：
Frey, William H., II
影响因子：
3.8
作者：
Driscoll, KE;Costa, DL;Schlesinger, RB
通讯作者：
Schlesinger, RB
DOI：
10.1152/ajplung.00173.2001
发表时间：
2002-04-01
影响因子：
4.9
作者：
Southam, DS;Dolovich, M;Inman, MD
通讯作者：
Inman, MD