The role of miR-130a-3p and SPOCK1 in tobacco exposed bronchial epithelial BEAS-2B transformed cells: Comparison to A549 and H1299 lung cancer cell lines
The role of miR-130a-3p and SPOCK1 in tobacco exposed bronchial epithelial BEAS-2B transformed cells: Comparison to A549 and H1299 lung cancer cell lines
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miR-130a-3p 和 SPOCK1 在烟草暴露的支气管上皮 BEAS-2B 转化细胞中的作用：与 A549 和 H1299 肺癌细胞系的比较
DOI：
10.1080/15287394.2019.1664479
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发表时间：
2019-08
期刊：
影响因子：
--
通讯作者：
Li Jianxiang
中科院分区：
文献类型：
--
作者：
Yu Xiao Fan;Wang Jin;Ouyang Nan;Guo Shuang;Sun Huiying;Tong Jian;Chen Tao;Li Jianxiang
ABSTRACT In the pathogenesis of human lung cancer induced by tobacco smoke decreased expression levels of microRNAs (miRNAs) are known to occur. At present, the specific miRNAs expression levels reduced by tobacco smoke and subsequent lung cellular transformation remain to be determined. The aim of this study was thus to identify the miRNAs affected following cigarette-smoke exposure in bronchial epithelial BEAS-2B cells that were malignantly transformed into S30 cells. In addition, the miRNAs in S30 transformed cells were compared to human lung cancer cell lines A549 and H1299. Our results identified miR-130a-3p which was down-regulated in S30 cells as well as A549 and H1299 lung cancer cell lines. Using miRNA mimic, a correlation between elevated miR-130a-3p expression levels and reduced migration in A549 and H1299 cell lines and S30 cells was noted as evidenced by transwell and wound healing assays accompanied by enhanced apoptosis. Further, two online target genes prediction programs TargetScan and miRDB were employed to identify the miRNA target gene SPOCK1 in all three cell types. SPOCK1 expression was higher in unexposed bronchial epithelial BEAS-2B cells. It is of interest that however silencing SPOCK1 in transformed S30 cells exposed to cigarette-smoke a marked depression in cell migration was noted. Our findings demonstrate that upregulated miR-130a-3p was associated with reduced SPOCK1 expression in transformed S30 as well as lung cancer A549 and H1299 cell lines indicating that cigarette transformed cells behave similar to lung cancer cells and this process involves diminished lung cancer cells migration.
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DOI：
10.7314/apjcp.2014.15.3.1391
发表时间：
2014-02
期刊：
Asian Pacific journal of cancer prevention : APJCP
影响因子：
--
作者：
Yong-ming Zhou;Juan Liu;Wei Sun
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Yong-ming Zhou;Juan Liu;Wei Sun
影响因子：
4.6
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DOI：
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发表时间：
2001-06
期刊：
Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego
影响因子：
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作者：
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I. Damps;E. Jassem;A. Siemińska
影响因子：
3.9
作者：
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DOI：
10.1007/978-1-61779-207-6_2
发表时间：
2011-01-01
期刊：
CELL MIGRATION: DEVELOPMENTAL METHODS AND PROTOCOLS, SECOND EDITION
影响因子：
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作者：
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Cory, Giles