Repeatability of exercise behaviors in mice.
Repeatability of exercise behaviors in mice.
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DOI：
10.1016/j.physbeh.2009.07.006
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发表时间：
2009-10-19
影响因子：
2.9
通讯作者：
Lightfoot, J. Timothy
中科院分区：
文献类型：
--
作者：
Knab, Amy M.;Bowen, Robert S.;Moore-Harrison, Trudy;Hamilton, Alicia Trynor;Turner, Michael J.;Lightfoot, J. Timothy
文献中经常使用啮齿动物运动行为的测量，例如最大跑步机耐力和体力活动；然而，关于这些运动行为测量的可重复性的可用数据很少。这项研究评估了常用的最大运动耐力跑步机测试的可重复性，以及通过小鼠轮跑测量的自愿体力活动。除了使用 Balb/cJ 小鼠 (n=20) 进行 10 周重复性分析外，还对近交系和远交系小鼠的跑步机测试的重复性进行了分析。通过轮跑（WR）的距离、持续时间和速度来评估自愿的日常身体活动。比较了一大群近交系和远交系小鼠 (n=739) 在 WR 第 5 天和第 6 天的体力活动测量结果。 Balb/cJ 和 DBA/2J 小鼠的不同组之间的运动耐力没有发现显着差异（p>0.05），这表明品系总体上测试相同；然而，BaD2F2 动物中的测试之间存在显着差异 (p<0.001)。 Bland-Altman 分析显示小鼠每周耐力测试之间缺乏一致性，相关性分析显示小鼠每周耐力测试之间缺乏一致的相关性。几天之间小鼠体内 WR 测量值没有发现显着差异 (p=0.99)。发现小鼠体内 WR 的天数之间存在高度相关性 (r=0.74–0.85)。重复耐力测试之间的小鼠内部高变异性表明，在带有冲击网格的封闭室中进行跑步机测试以激发小鼠跑步的动机是不可重复的。相反，轮跑测量天数之间的高度相关性和一致性表明，随意活动（WR）在个体小鼠中是可重复和稳定的。
Measurements of exercise behaviors in rodents such as maximal treadmill endurance and physical activity are often used in the literature; however, minimal data are available regarding the repeatability of measurements used for these exercise behaviors. This study assessed the repeatability of a commonly used maximal exercise endurance treadmill test as well as voluntary physical activity measured by wheel running in mice. Repeatability of treadmill tests were analyzed for both inbred and outbred mice in addition to a 10 week repeatability analysis using Balb/cJ mice (n=20). Voluntary daily physical activity was assessed by distance, duration, and speed of wheel running (WR). Physical activity measurements on days 5 and 6 of WR in a large cohort (n=739) of both inbred and outbred mice were compared. No significant differences (p>0.05) in exercise endurance were found between different cohorts of Balb/cJ and DBA/2J mice indicating strains overall generally test the same; however, significant differences between tests were seen within BaD2F2 animals (p<0.001). Bland-Altman analysis revealed lack of agreement between weekly endurance tests within mouse, and correlation analysis showed lack of consistent correlations between weekly endurance tests within mouse. No significant differences were found for WR measurements within mouse between days (p=0.99). High correlations between days within mouse for WR were found (r=0.74–0.85). High intra-mouse variability between repeated endurance tests suggests that treadmill testing in an enclosed chamber with shock grid for motivation to run in mice is not repeatable. Conversely, high correlation and agreement between days of wheel running measurements suggest that voluntary activity (WR) is repeatable and stable within individual mice.
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影响因子：
64.5
作者：
Narkar VA;Downes M;Yu RT;Embler E;Wang YX;Banayo E;Mihaylova MM;Nelson MC;Zou Y;Juguilon H;Kang H;Shaw RJ;Evans RM
通讯作者：
Evans RM
DOI：
10.1097/00005768-200112000-00012
发表时间：
2001-12-01
期刊：
MEDICINE AND SCIENCE IN SPORTS AND EXERCISE
影响因子：
--
作者：
Lightfoot, JT;Turner, MJ;Kleeberger, SR
通讯作者：
Kleeberger, SR
影响因子：
3.3
作者：
Lightfoot, J. Timothy;Turner, Michael J.;Kleeberger, Steven R.
通讯作者：
Kleeberger, Steven R.
影响因子：
3.3
作者：
COYLE, EF;COGGAN, AR;WALTERS, TJ
通讯作者：
WALTERS, TJ
影响因子：
3.3
作者：
Lerman, I;Harrison, BC;Leinwand, LA
通讯作者：
Leinwand, LA