Antibacterial and antioxidant properties of the methanol extracts of the leaves and stems of Calpurnia aurea.
Antibacterial and antioxidant properties of the methanol extracts of the leaves and stems of Calpurnia aurea.
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卡尔浦尼香肠叶和茎的甲醇提取物的抗菌和抗氧化特性。
DOI：
10.1186/1472-6882-8-53
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发表时间：
2008-09-20
影响因子：
--
通讯作者：
Masika, Patrick J.
中科院分区：
文献类型：
--
作者：
Adedapo, Adeolu A.;Jimoh, Florence O.;Koduru, Srinivas;Afolayan, Anthony J.;Masika, Patrick J.
在南非，Calpurnia aurea (Ait.) Benth 用于消灭虱子、止痒、消灭蛆虫和治疗过敏性皮疹，特别是由毛毛虫引起的皮疹。抗氧化剂在防止活性氧损伤方面发挥着重要作用。据报道，含有类黄酮的植物具有很强的抗氧化特性。采用体外标准方法评价了 Calpurnia aurea 叶和茎的甲醇提取物的抗菌、抗氧化活性和酚含量。分光光度法是测定总酚、总黄酮、黄酮醇和原花青素的基础。这些参数使用单宁、槲皮素和儿茶素等价物。采用ABTS、DPPH和亚铁还原抗氧化性能（FRAP）方法测定了Calpurnia aurea茎提取物的抗氧化活性。使用实验室分离的 10 种细菌（包括 5 种革兰氏阳性菌株和 5 种革兰氏阴性菌株）来测定该植物的抗菌活性。本研究结果表明，通过总酚、黄酮和FRAP方法测定，Calpurnia aurea茎提取物的抗氧化活性高于叶子。另一方面，该植物的叶提取物具有较高水平的总黄酮醇和原花青素。叶提取物还具有较高的自由基清除活性，如 1, 1-二苯基-2-三硝基苯肼 (DPPH) 和 2,2'-嗪双-3-乙基苯并噻唑啉-6-磺酸 (ABTS) 测定所示。叶子提取物显示出对抗七种细菌的活性。这项研究的结果表明，Calpurnia aurea 的叶和茎提取物具有抗氧化特性，可以作为自由基抑制剂或清除剂，或者可能作为主要抗氧化剂。虽然，Calpurnia aurea 的抗菌特性不如标准药物氯霉素和链霉素有效，但它们仍然对本研究中使用的细菌菌株具有一定的活性。因此，Calpurnia aurea 可能是功能性食品以及植物性药物产品的良好候选者。
In South Africa, Calpurnia aurea (Ait.) Benth is used to destroy lice and to relieve itches, to destroy maggots and to treat allergic rashes, particularly those caused by caterpillars. Antioxidants play an important role protecting against damage by reactive oxygen species. Plants containing flavonoids have been reported to possess strong antioxidant properties. The antibacterial, antioxidant activities and phenolic contents of the methanol extracts of the leaves and stems of Calpurnia aurea were evaluated using in vitro standard methods. Spectrophotometry was the basis for the determinations of total phenol, total flavonoids, flavonols, and proanthocyanidins. Tannins, quercetin and catechin equivalents were used for these parameters. The antioxidant activities of the stem extract of Calpurnia aurea were determined by ABTS, DPPH, and ferrous reducing antioxidant property (FRAP) methods. Laboratory isolates of 10 bacteria species which included five Gram-positive and five Gram-negative strains were used to assay for antibacterial activity of this plant. The results from this study showed that the antioxidant activities of the stem extract of Calpurnia aurea as determined by the total phenol, flavonoids, and FRAP methods were higher than that of the leaves. On the other hand, the leaf extract of the plant has higher level of total flavonols and proanthocyanidins. The leaf extract also has higher radical scavenging activity as shown in 1, 1-Diphenyl-2-picrylhydrazyl (DPPH), and 2,2¿-azinobis-3- ethylbenzothiazoline-6-sulfonic acid (ABTS) assay. The leaf extract showed activity against seven of the bacterial organisms. The results from this study indicate that the leaves and stem extracts of Calpurnia aurea possess antioxidant properties and could serve as free radical inhibitors or scavenger or, acting possibly as primary antioxidants. Although, the antibacterial properties of Calpurnia aurea are not as effective as the standard drugs- Chloramphenicol and Streptomycin, they still possess some activity against bacterial strains used in this study. Calpurnia aurea may therefore be a good candidate for functional foods as well as pharmaceutical plant-based products.
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影响因子：
3.8
作者：
ASRES, K;GIBBONS, WA;MASCAGNI, P
通讯作者：
MASCAGNI, P
影响因子：
5.4
作者：
Badami, S;Gupta, MK;Suresh, B
通讯作者：
Suresh, B
影响因子：
5.4
作者：
Grierson, DS;Afolayan, AJ
通讯作者：
Afolayan, AJ
影响因子：
6
作者：
Behera, BC;Verma, N;Makhija, U
通讯作者：
Makhija, U
影响因子：
4.3
作者：
Mathew, S;Abraham, TE
通讯作者：
Abraham, TE