Staphylococcus aureus Bacteriophage Suppresses LPS-Induced Inflammation in MAC-T Bovine Mammary Epithelial Cells.
Staphylococcus aureus Bacteriophage Suppresses LPS-Induced Inflammation in MAC-T Bovine Mammary Epithelial Cells.
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金黄色葡萄球菌噬菌体抑制 MAC-T 牛乳腺上皮细胞中 LPS 诱导的炎症。
DOI：
10.3389/fmicb.2018.01614
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发表时间：
2018
影响因子：
5.2
通讯作者：
Wang R
中科院分区：
文献类型：
--
作者：
Zhang L;Hou X;Sun L;He T;Wei R;Pang M;Wang R
先前的几项研究表明，噬菌体可以显著影响各种细胞因子的产生。本研究的目的是用实时聚合酶链式反应(PCR)和免疫印迹法研究VBSAUMJS25噬菌体对刺激的MAC-T牛乳腺上皮细胞的炎症作用及其机制。实验表明，VB_SAUM_JS25可降低金黄色葡萄球菌或脂多糖诱导的MACT细胞肿瘤坏死因子-α、白介素1、白介素6、白介素8、白介素10的水平，并调节正常T细胞的活化、正常T细胞表达和分泌(RANTES)α，这种作用可能与其抗菌作用无关。此外，金黄色葡萄球菌噬菌体VBSAUMJS25可抑制脂多糖诱导的核因子-κBp65的磷酸化，这可能是其作用的重要机制之一。必须在噬菌体的激活和抑制之间保持仔细调节的平衡，以避免不适当的炎症反应。VB_SAUM_JS25对免疫应答的影响突出了噬菌体治疗的潜在发展和应用前景，并可能代表一种新的抗炎治疗策略。
Several previous studies have shown that bacteriophages can significantly affect the production of various cytokines. The aim of this present study was to investigate the inflammatory effects and mechanisms of bacteriophage vB_SauM_JS25 in stimulated MAC-T bovine mammary epithelial cells by real-time polymerase chain reaction (PCR) and Western blotting. Experiments show that vB_SauM_JS25 reduces Staphylococcus aureus- or lipopolysaccharide (LPS)-induced levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, IL-8, IL-10, and regulated on activation, normal T cell expressed and secreted (RANTES) mRNA in MAC-T cells, in a manner expected to be unrelated to its antibacterial action. Moreover, S. aureus bacteriophage vB_SauM_JS25 suppressed the LPS-induced phosphorylation of nuclear factor (NF)-κB p65, which may represent an important mechanism mediating these effects. A carefully regulated balance between activation and inhibition by bacteriophages must be kept avoiding inappropriate inflammatory responses. The ability of vB_SauM_JS25 to influence the immune response highlights the potential development and application of bacteriophage-based therapies and may represent a novel anti-inflammatory therapeutic strategy.
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影响因子：
4.2
作者：
Zimecki M;Artym J;Kocieba M;Weber-Dabrowska B;Borysowski J;Górski A
通讯作者：
Górski A
影响因子：
4.4
作者：
Gilbert FB;Cunha P;Jensen K;Glass EJ;Foucras G;Robert-Granié C;Rupp R;Rainard P
通讯作者：
Rainard P
影响因子：
3.7
作者：
Zbinden C;Stephan R;Johler S;Borel N;Bünter J;Bruckmaier RM;Wellnitz O
通讯作者：
Wellnitz O
影响因子：
20.3
作者：
Elson, Greg;Dunn-Siegrist, Irene;Pugin, Jerome
通讯作者：
Pugin, Jerome
影响因子：
2.9
作者：
REDDY, KJ;KUWABARA, T;SHERMAN, LA
通讯作者：
SHERMAN, LA