The inflammatory response of primary bovine mammary epithelial cells to Staphylococcus aureus strains is linked to the bacterial phenotype.
The inflammatory response of primary bovine mammary epithelial cells to Staphylococcus aureus strains is linked to the bacterial phenotype.
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DOI：
10.1371/journal.pone.0087374
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Wellnitz O
中科院分区：
文献类型：
--
作者：
Zbinden C;Stephan R;Johler S;Borel N;Bünter J;Bruckmaier RM;Wellnitz O
金黄色葡萄球菌是引起奶牛乳腺炎的主要病原体。基于胶乳凝集的金黄色葡萄球菌试验可将牛金黄色葡萄球菌菌株分为胶乳凝集试验（SLAT）阴性[SLAT(−)]和SLAT阳性[SLAT(+)]分离株。SLAT（−）分离株的毒力和抗性基因谱高度相似，但与SLAT（+）分离株有很大差异。值得注意的是，在SLAT（−）分离株中检测到重要毒力因子的特异性遗传变化。基于分子数据，我们假设SLAT（+）菌株比SLAT（−）菌株更具毒力。本研究的目的是研究SLAT（−）和SLAT（+）菌株对乳腺上皮细胞的粘附能力是否能诱导不同的免疫反应，进而在乳腺炎的过程中发挥作用。用从临床牛乳腺炎病例中分离的热灭活SLAT(+) （n = 3）和SLAT(-) （n = 3）菌株的悬液攻击原代牛乳腺上皮细胞（bMEC）。1、6和24 h后，收集细胞，通过反转录和定量PCR检测炎症介质（TNF-α、IL-1β、IL-8、RANTES、SAA、乳铁蛋白、GM-CSF、COX-2和TLR-2）的mRNA表达。大多数被测因子的转录（ΔΔCT）在攻毒的bMEC中被诱导了6和24 h。有趣的是，与SLAT（-）菌株相比，SLAT（+）菌株的相对mRNA水平更高（P<0.05）。此外，SLAT（+）和SLAT（−）菌株对bMEC的粘附实验也显示出显著差异。本研究清楚地表明，这两种金黄色葡萄球菌菌株类型引起bMEC的不同免疫反应，并且在体外表现出不同的粘附能力。这可能反映了不同菌株类型可能引起的乳腺炎严重程度的差异。
Staphylococcus aureus is a major mastitis-causing pathogen in dairy cows. The latex agglutination-based Staphaurex test allows bovine S. aureus strains to be grouped into Staphaurex latex agglutination test (SLAT)-negative [SLAT(−)] and SLAT-positive [SLAT(+)] isolates. Virulence and resistance gene profiles within SLAT(−) isolates are highly similar, but differ largely from those of SLAT(+) isolates. Notably, specific genetic changes in important virulence factors were detected in SLAT(−) isolates. Based on the molecular data, it is assumed that SLAT(+) strains are more virulent than SLAT(−) strains. The objective of this study was to investigate if SLAT(−) and SLAT(+) strains can differentially induce an immune response with regard to their adhesive capacity to epithelial cells in the mammary gland and in turn, could play a role in the course of mastitis. Primary bovine mammary epithelial cells (bMEC) were challenged with suspensions of heat inactivated SLAT(+) (n = 3) and SLAT(−) (n = 3) strains isolated from clinical bovine mastitis cases. After 1, 6, and 24 h, cells were harvested and mRNA expression of inflammatory mediators (TNF-α, IL-1β, IL-8, RANTES, SAA, lactoferrin, GM-CSF, COX-2, and TLR-2) was evaluated by reverse transcription and quantitative PCR. Transcription (ΔΔCT) of most measured factors was induced in challenged bMEC for 6 and 24 h. Interestingly, relative mRNA levels were higher (P<0.05) in response to SLAT(+) compared to SLAT(−) strains. In addition, adhesion assays on bMEC also showed significant differences between SLAT(+) and SLAT(−) strains. The present study clearly shows that these two S. aureus strain types cause a differential immune response of bMEC and exhibit differences in their adhesion capacity in vitro. This could reflect differences in the severity of mastitis that the different strain types may induce.
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DOI：
10.1016/0147-9571(86)90072-x
发表时间：
1986-01-01
影响因子：
2
作者：
CRAVEN, N
通讯作者：
CRAVEN, N
影响因子：
3.5
作者：
Griesbeck-Zilch, B.;Meyer, H. H. D.;Wellnitz, O.
通讯作者：
Wellnitz, O.
DOI：
10.1046/j.1439-0450.2003.00628.x
发表时间：
2003-04-01
期刊：
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH
影响因子：
--
作者：
Alluwaimi, AM;Leutenegger, CM;Cullor, JS
通讯作者：
Cullor, JS
影响因子：
3.3
作者：
Bannerman, D. D.
通讯作者：
Bannerman, D. D.
影响因子：
3.5
作者：
Griesbeck-Zilch, B.;Osman, M.;Wellnitz, O.
通讯作者：
Wellnitz, O.