Acrylic acid surface-modified contact lens for the culture of limbal stem cells.
Acrylic acid surface-modified contact lens for the culture of limbal stem cells.
复制标题
用于培养角膜缘干细胞的丙烯酸表面改性隐形眼镜。
作者：
Hong Zhang;K. Brown;Sue Peng Lowe;Guei;David Steele;K. Abberton;M. Daniell
对生物材料表面的表面处理已被证明可以改变和帮助细胞生长。我们的目的是确定用于治疗角膜缘干细胞缺乏症（LSCD）的最佳表面修饰系统，该系统将促进自体角膜缘上皮细胞的扩增，同时保持培养的上皮细胞处于分化程度较低的状态。通过等离子体聚合对市售接触透镜（CL）进行各种表面改性，其中在100%丙烯酸、50：50%丙烯酸：辛酸和100%辛酸下测试丙烯酸与辛酸的比率，以产生高酸、中等酸和无酸。使用X射线光电子能谱分析等离子体聚合物沉积层的化学组成。在高酸修饰的CL上培养的角膜缘外植体长出更多的细胞。免疫荧光和RT 2-PCR阵列结果表明，较高的丙烯酸含量也有助于在上皮细胞的离体扩增期间维持祖细胞。本研究首次证明了高酸修饰的CL能够保持干细胞性，并可作为培养角膜缘细胞的基质用于治疗LSCD。
Surface treatment to a biomaterial surface has been shown to modify and help cell growth. Our aim was to determine the best surface-modified system for the treatment of limbal stem cell deficiency (LSCD), which would facilitate expansion of autologous limbal epithelial cells, while maintaining cultivated epithelial cells in a less differentiated state. Commercially available contact lenses (CLs) were variously surface modified by plasma polymerization with ratios of acrylic acid to octadiene tested at 100% acrylic acid, 50:50% acrylic acid:octadiene, and 100% octadiene to produce high-, mid-, and no-acid. X-ray photoelectron spectroscopy was used to analyze the chemical composition of the plasma polymer deposited layer. Limbal explants cultured on high acid-modified CLs outgrew more cells. Immunofluorescence and RT2-PCR array results indicated that a higher acrylic acid content can also help maintain progenitor cells during ex vivo expansion of epithelial cells. This study provides the first evidence for the ability of high acid-modified CLs to preserve the stemness and to be used as substrates for the culture of limbal cells in the treatment of LSCD.
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影响因子：
4
作者：
M. Levrero;V. Laurenzi;A. Costanzo;J. Gong;J. Wang;G. Melino
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M. Levrero;V. Laurenzi;A. Costanzo;J. Gong;J. Wang;G. Melino
影响因子：
14
作者：
Yim, EKF;Reano, RM;Leong, KW
通讯作者：
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影响因子：
10.5
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Koster, MI;Kim, S;Roop, DR
通讯作者：
Roop, DR
影响因子：
4.4
作者：
Nakatsu, Martin N.;Ding, Zhenhua;Deng, Sophie X.
通讯作者：
Deng, Sophie X.
影响因子：
6.2
作者：
Lawrence, Brian D.;Cronin-Golomb, Mark;Omenetto, Fiorenzo G.
通讯作者：
Omenetto, Fiorenzo G.