A QuEChERS-Based Liquid Chromatography-Tandem Mass Spectrometry Method for the Simultaneous Determination of Nine Zearalenone-Like Mycotoxins in Pigs.
A QuEChERS-Based Liquid Chromatography-Tandem Mass Spectrometry Method for the Simultaneous Determination of Nine Zearalenone-Like Mycotoxins in Pigs.
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基于 QuEChERS 的液相色谱-串联质谱法同时测定猪体内九种玉米赤霉烯酮类霉菌毒素
DOI：
10.3390/toxins10030129
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发表时间：
2018-03-20
期刊：
影响因子：
4.2
通讯作者：
Wu A
中科院分区：
文献类型：
--
作者：
Yan Z;Wang L;Wang J;Tan Y;Yu D;Chang X;Fan Y;Zhao D;Wang C;De Boevre M;De Saeger S;Sun C;Wu A
玉米赤霉烯酮（ZEN）及其衍生物作为生物标志物在动物组织或器官中的检测在霉菌毒素监测中起着重要的作用，可以促进有效的暴露评估。建立了液相色谱-串联质谱（LC-MS/MS）同时定量猪不同组织（心脏、肝脏、脾脏和肌肉）中9种禅宗样真菌毒素（包括3种葡萄糖醛酸酯）的方法，并对其进行了验证。组织样品采用快速、简便、廉价、有效、坚固、安全的（QuEChERS）提取和清理程序进行提取，并采用多反应监测（MRM）模式进行LC-MS/MS分析。各目标分析物的动态线性范围为R2在0.916 ~ 0.999之间。6种ZENs的lod范围为0.5 ~ 1 ng/g， loq范围为1 ~ 2 ng/g。日内、日间重现性良好（RSDr和RSDr均< 20%），表明该方法具有良好的稳定性。9种目标分析物的加样回收率在70 ~ 110%之间。验证结果表明，LC-MS/MS联用QuEChERS样品前处理方法有效，适用于猪体内ZEN代谢物的同时定量。本研究已将其应用于猪组织的分析，也可适用于霉菌毒素研究领域的样品分析。
The determination of zearalenone (ZEN) and its derivatives as biomarkers in animal tissues or organs plays an important role in mycotoxin monitoring and can promote effective exposure assessment. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous quantification of nine ZEN-like mycotoxins, including three glucuronides in different pig tissues (heart, liver, spleen and muscle) was developed and validated in this study. Tissue samples were extracted using a quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction and clean-up procedure, and analyzed by LC-MS/MS in multiple reaction monitoring (MRM) mode. Dynamic linear ranges for each target analyte were determined with R2 between 0.916 and 0.999. The LODs of the six ZENs were achieved in the range of 0.5–1 ng/g and the LOQs varied from 1 ng/g to 2 ng/g. The satisfying intra-day and inter-day reproducibility (both RSDr and RSDR < 20%) indicated a good stability of this method. The recoveries of the nine target analytes were in the range of 70–110%. The validation results showed that this LC-MS/MS method coupled with QuEChERS sample pretreatment is effective and suitable for the simultaneous quantitation of ZEN metabolites in pigs. It has been applied to analysis of the pig tissues in this research and can be also adapted for samples in the mycotoxin research field.
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影响因子：
8.8
作者：
Belhassen, H.;Jimenez-Diaz, I.;Hedili, A.
通讯作者：
Hedili, A.
影响因子：
2.8
作者：
Gajecka, Magdalena;Slawuta, Piotr;Nicpon, Jozef
通讯作者：
Nicpon, Jozef
影响因子：
2
作者：
Gromadzka, K.;Waskiewicz, A.;Golinski, P.
通讯作者：
Golinski, P.
影响因子：
6.1
作者：
Dzuman, Zbynek;Zachariasova, Milena;Hajslova, Jana
通讯作者：
Hajslova, Jana
影响因子：
4.2
作者：
Marin DE;Motiu M;Taranu I
通讯作者：
Taranu I