Food contaminant zearalenone and its metabolites affect cytokine synthesis and intestinal epithelial integrity of porcine cells.
Food contaminant zearalenone and its metabolites affect cytokine synthesis and intestinal epithelial integrity of porcine cells.
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DOI：
10.3390/toxins7061979
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发表时间：
2015-05-29
期刊：
影响因子：
4.2
通讯作者：
Taranu I
中科院分区：
文献类型：
--
作者：
Marin DE;Motiu M;Taranu I
肠上皮是抵抗食物污染物的第一道屏障。玉米赤霉烯酮（ZEN）是一种雌激素性真菌毒素，是谷物、食品和饲料中常见的污染物。在本研究中，我们研究了浓度为10-100 µM的ZEN及其某些代谢产物（α-ZOL、β-ZOL）对猪上皮细胞系：猪肠上皮细胞（IPEC-1）的体外作用。我们证明，XTT试验结果表明，两种ZEN代谢物对IPEC细胞的毒性更大，而中性红试验结果表明，对于低于10 µM的剂量，只有β-ZOL显示出比上皮细胞更明显的细胞毒性。ZEN对TER值没有影响，而α-ZOL从治疗第4天开始显著降低TER值。β-ZOL具有双重作用，首先它诱导TER显著增加，然后，从第6天开始，它诱导TER值与第0天相比显著降低。关于细胞因子的合成，我们的结果表明，ZEN有增加IL-8和IL-10合成的趋势。相反，α-和β-ZOL以剂量依赖性方式降低IL-8和IL-10的表达。总之，我们的研究结果表明，ZEN及其代谢产物不同地影响猪肠道细胞活力，跨上皮阻力和细胞因子合成，对肠道健康具有重要意义。
The intestinal epithelium is the first barrier against food contaminants. Zearalenone (ZEN) is an estrogenic mycotoxin that was identified as a common contaminant of cereal grains and food and feedstuffs. In the present study, we have investigated the in vitro effects of ZEN and some of its metabolites (α-ZOL, β-ZOL) in concentrations of 10–100 µM on a swine epithelial cell line: Intestinal porcine epithelial cells (IPEC-1). We demonstrated that both ZEN metabolites were more toxic for IPEC cells as resulted from the XTT test, while for doses lower than 10 µM, only β-ZOL showed a more pronounced cytotoxicity versus epithelial cells as resulted from neutral red assay. ZEN has no effect on TER values, while α-ZOL significantly decreased the TER values, starting with day 4 of treatment. β-ZOL had a dual effect, firstly it induced a significant increase of TER, and then, starting on day 6, it induced a dramatic decrease of TER values as compared with on day 0. Concerning the cytokine synthesis, our results showed that ZEN has a tendency to increase the synthesis of IL-8 and IL-10. By contrast, α- and β-ZOL decreased the expression of both IL-8 and IL-10, in a dose dependent manner. In conclusion, our results showed that ZEN and its metabolites differently affected porcine intestinal cell viability, transepithelial resistance and cytokine synthesis with important implication for gut health.
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影响因子：
14.2
作者：
Groschwitz, Katherine R.;Hogan, Simon P.
通讯作者：
Hogan, Simon P.
影响因子：
3.8
作者：
Bouhet, S;Hourcade, E;Oswald, IP
通讯作者：
Oswald, IP
影响因子：
4.3
作者：
OLSEN, M;PETTERSSON, H;KIESSLING, KH
通讯作者：
KIESSLING, KH
影响因子：
3.7
作者：
Osselaere A;Santos R;Hautekiet V;De Backer P;Chiers K;Ducatelle R;Croubels S
通讯作者：
Croubels S
影响因子：
1.2
作者：
Dong, Mei;Tulayakul, Phitsanu;Kumagai, Susumu
通讯作者：
Kumagai, Susumu