Metabolomics analysis reveals Embden Meyerhof Parnas pathway activation and flavonoids accumulation during dormancy transition in tree peony.
Metabolomics analysis reveals Embden Meyerhof Parnas pathway activation and flavonoids accumulation during dormancy transition in tree peony.
复制标题
代谢组学分析揭示牡丹休眠过渡期间 Embden Meyerhof Parnas 通路激活和黄酮类化合物积累
DOI：
10.1186/s12870-020-02692-x
复制
发表时间：
2020-10-23
影响因子：
5.3
通讯作者：
Gai S
中科院分区：
文献类型：
--
作者：
Zhang T;Yuan Y;Zhan Y;Cao X;Liu C;Zhang Y;Gai S
研究背景芽休眠是植物在坚韧中生存的一种复杂策略。休眠是牡丹反季节栽培的主要障碍，充分的低温暴露是促进牡丹等多年生植物休眠解除的有效方法。结果对牡丹芽进行人工低温处理，在0-4 °C处理0、7、14、21和28 d后的5个时间点进行代谢组学分析。共分离得到535个代谢产物，分为11类，包括黄酮类、氨基酸及其衍生物、脂类、有机酸及其衍生物、核苷酸及其衍生物、生物碱、羟基肉桂酸衍生物、碳水化合物和醇类、植物激素、香豆素类和维生素类。共检测到118种差异代谢产物（VIP ≥ 1，P<0. 05），其KEGG途径参与了多条与休眠相关的代谢途径。蔗糖是牡丹花蕾中含量最丰富的碳水化合物。在休眠解除过程中，淀粉降解，EMP活性增加，这与糖含量、相关酶活性和关键基因表达的变化有关。长时间低温也促进了风味物质的合成和积累。此外，植物激素（水杨酸，茉莉酸，脱落酸，吲哚-3-乙酸）的变化表明，他们发挥了不同的作用在休眠transition.ConclusionOur研究表明，淀粉降解，EMP激活，黄酮类化合物的积累是至关重要的，并与牡丹芽休眠过渡。
BackgroundBud dormancy is a sophisticated strategy which plants evolve to survive in tough environments. Endodormancy is a key obstacle for anti-season culture of tree peony, and sufficient chilling exposure is an effective method to promote dormancy release in perennial plants including tree peony. However, the mechanism of dormancy release is still poorly understood, and there are few systematic studies on the metabolomics during chilling induced dormancy transition.ResultsThe tree peony buds were treated with artificial chilling, and the metabolmics analysis was employed at five time points after 0–4 °C treatment for 0, 7, 14, 21 and 28 d, respectively. A total of 535 metabolites were obtained and devided into 11 groups including flavonoids, amino acid and its derivatives, lipids, organic acids and its derivates, nucleotide and its derivates, alkaloids, hydroxycinnamoyl derivatives, carbohydrates and alcohols, phytohormones, coumarins and vitamins. Totally, 118 differential metabolites (VIP ≥ 1,P< 0.05) during chilling treatment process were detected, and their KEGG pathways involved in several metabolic pathways related to dormancy. Sucrose was the most abundant carbohydrate in peony bud. Starch was degradation and Embden Meyerhof Parnas (EMP) activity were increased during the dormancy release process, according to the variations of sugar contents, related enzyme activities and key genes expression. Flavonoids synthesis and accumulation were also promoted by prolonged chilling. Moreover, the variations of phytohormones (salicylic acid, jasmonic acid, abscisic acid, and indole-3-acetic acid) indicated they played different roles in dormancy transition.ConclusionOur study suggested that starch degradation, EMP activation, and flavonoids accumulation were crucial and associated with bud dormancy transition in tree peony.
登录
查看更多内容
影响因子：
4.3
作者：
Kosova, Klara;Prasil, Ilja Tom;Vankova, Radomira
通讯作者：
Vankova, Radomira
影响因子：
5.6
作者：
Ionescu IA;López-Ortega G;Burow M;Bayo-Canha A;Junge A;Gericke O;Møller BL;Sánchez-Pérez R
通讯作者：
Sánchez-Pérez R
一种用于大规模检测、鉴定和定量广泛目标代谢物的新型综合方法：在水稻代谢组学研究中的应用。
影响因子：
27.5
作者：
Chen, Wei;Gong, Liang;Luo, Jie
通讯作者：
Luo, Jie
影响因子：
7.4
作者：
Dahlke, Renate I.;Fraas, Simon;Steffens, Bianka
通讯作者：
Steffens, Bianka
影响因子：
4
作者：
Bonhomme, Marc;Peuch, Mederic;Lacointe, Andre
通讯作者：
Lacointe, Andre