Mechanism of electroacupuncture and herb-partitioned moxibustion on ulcerative colitis animal model: A study based on proteomics.
Mechanism of electroacupuncture and herb-partitioned moxibustion on ulcerative colitis animal model: A study based on proteomics.
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DOI：
10.3748/wjg.v28.i28.3644
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发表时间：
2022-07-28
影响因子：
4.3
通讯作者：
中科院分区：
文献类型：
--
作者：
溃疡性结肠炎（UC）是一种慢性、非特异性肠道炎症性疾病。针灸治疗溃疡性结肠炎已被证实有效，但其作用机制尚不清楚。蛋白质组学技术揭示了多种与UC免疫、炎症相关的生物学标志物，为针灸治疗UC的机制研究提供了新的思路和方向。应用蛋白质组学技术研究电针和隔药灸治疗UC大鼠的作用机制。雄性SD大鼠随机分为正常组（N组）、葡聚糖硫酸钠（DSS）诱导的UC模型组（M组）、HM组和EA组。用3%DSS制备UC大鼠模型，HM组和EA组分别在双侧天枢、气海穴进行HM和EA干预。苏木精和伊红染色用于结肠组织的形态学评价。采用同位素标记相对和绝对定量（iTRAQ）和液相色谱-串联质谱（LC-MS）技术对结肠组织进行蛋白质组分析，并对各组间差异表达蛋白进行生物信息学分析和蛋白质相互作用网络构建。然后用western blot对筛选出的DEPs进行验证。与M组相比，HM组和EA组大鼠的结肠损伤评分和组织病理学评分均显著降低（P < 0.01）。与N组相比，M组共检测到202个DEPs，其中111个蛋白表达上调，91个蛋白表达下调，分别有25个和15个蛋白在HM和EA干预后被逆转。DEPs参与多种生物学过程，如生物学调节、免疫系统进展以及多种途径，包括自然杀伤细胞介导的细胞毒性、肠道免疫网络产生免疫球蛋白A（伊加）和Fcγ R介导的吞噬作用。京都基因和基因组百科全书中HM组和M组、EA组和M组之间的DEPs途径均包括免疫相关和氧化磷酸化。网络分析显示，各组DEPs均参与了多条蛋白质-蛋白质相互作用途径，且氧化磷酸化途径相关蛋白ATP合成酶亚基g（ATP 5L）、ATP合成酶β亚基前体（Atp 5 f）、细胞色素c氧化酶亚基4亚型1（Cox 4 i1）的表达在HM和EA干预后均下调。选定DEP的后续验证（突触囊泡糖蛋白2A;核帽结合蛋白亚基1;氨甲酰磷酸合成酶1; Cox 4 i 1; ATP合成酶亚基B，Atp 5 f 1; doublecortin like kinase 3）蛋白质印迹法证实了iTRAQ数据的可靠性，HM和EA干预可显著下调氧化磷酸化相关蛋白的表达Cox 4 i1、Atp 5 f1与对照组比较差异有统计学意义（P < 0.01）。电针和HM可通过调节与氧化磷酸化相关的ATP 5L、Atp 5 f1、Cox 4 i1的表达，进而调节肠道免疫网络中伊加生成、Fcγ R介导的吞噬等免疫相关通路，从而减轻DSS诱导的UC大鼠结肠炎症。
Ulcerative colitis (UC) is a chronic, nonspecific intestinal inflammatory disease. Acupuncture and moxibustion is proved effective in treating UC, but the mechanism has not been clarified. Proteomic technology has revealed a variety of biological markers related to immunity and inflammation in UC, which provide new insights and directions for the study of mechanism of acupuncture and moxibustion treatment of UC. To investigate the mechanism of electroacupuncture (EA) and herb-partitioned moxibustion (HM) on UC rats by using proteomics technology. Male Sprague-Dawley rats were randomly divided into the normal (N) group, the dextran sulfate sodium (DSS)-induced UC model (M) group, the HM group, and the EA group. UC rat model was prepared with 3% DSS, and HM and EA interventions at the bilateral Tianshu and Qihai acupoints were performed in HM or EA group. Haematoxylin and eosin staining was used for morphological evaluation of colon tissues. Isotope-labeled relative and absolute quantification (iTRAQ) and liquid chromatography-tandem mass spectrometry were performed for proteome analysis of the colon tissues, followed by bioinformatics analysis and protein-protein interaction networks establishment of differentially expressed proteins (DEPs) between groups. Then western blot was used for verification of selected DEPs. The macroscopic colon injury scores and histopathology scores in the HM and EA groups were significantly decreased compared to the rats in the M group (P < 0.01). Compared with the N group, a total of 202 DEPs were identified in the M group, including 111 up-regulated proteins and 91 down-regulated proteins, of which 25 and 15 proteins were reversed after HM and EA interventions, respectively. The DEPs were involved in various biological processes such as biological regulation, immune system progression and in multiple pathways including natural killer cell mediated cytotoxicity, intestinal immune network for immunoglobulin A (IgA) production, and FcγR-mediated phagocytosis. The Kyoto Encyclopedia of Genes and Genomes pathways of DEPs between HM and M groups, EA and M groups both included immune-associated and oxidative phosphorylation. Network analysis revealed that multiple pathways for the DEPs of each group were involved in protein-protein interactions, and the expression of oxidative phosphorylation pathway-related proteins, including ATP synthase subunit g (ATP5L), ATP synthase beta subunit precursor (Atp5f), cytochrome c oxidase subunit 4 isoform 1 (Cox4i1) were down-regulated after HM and EA interventions. Subsequent verification of selected DEPs (Synaptic vesicle glycoprotein 2A; nuclear cap binding protein subunit 1; carbamoyl phosphate synthetase 1; Cox4i1; ATP synthase subunit b, Atp5f1; doublecortin like kinase 3) by western blot confirmed the reliability of the iTRAQ data, HM and EA interventions can significantly down-regulate the expression of oxidative phosphorylation-associated proteins (Cox4i1, Atp5f1) (P < 0.01). EA and HM could regulate the expression of ATP5L, Atp5f1, Cox4i1 that associated with oxidative phosphorylation, then might regulate immune-related pathways of intestinal immune network for IgA production, FcγR-mediated phagocytosis, thereby alleviating colonic inflammation of DSS-induced UC rats.
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影响因子：
3.6
作者：
Brenna Ø;Furnes MW;Munkvold B;Kidd M;Sandvik AK;Gustafsson BI
通讯作者：
Gustafsson BI
影响因子：
1.1
作者：
Lin, Ya-Ying;Zhao, Ji-Meng;Wu, Huan-Gan
通讯作者：
Wu, Huan-Gan
影响因子：
2
作者：
Brentnall, Teresa A.;Pan, Sheng;Bronner, Mary P.;Crispin, David A.;Mirzaei, Hamid;Cooke, Kelly;Tamura, Yasuko;Nikolskaya, Tatiana;JeBailey, Lellean;Goodlett, David R.;McIntosh, Martin;Aebersold, Ruedi;Rabinovitch, Peter S.;Chen, Ru
通讯作者：
Chen, Ru
影响因子：
4.6
作者：
Lo Sasso, Giuseppe;Phillips, Blaine W.;Hoeng, Julia
通讯作者：
Hoeng, Julia
隔药灸对 DSS 溃疡性结肠炎大鼠结肠 miRNA 表达谱的影响
DOI：
10.1155/2017/1767301
发表时间：
2017
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Huang Y;Ma Z;Cui YH;Dong HS;Zhao JM;Dou CZ;Liu HR;Li J;Wu HG
通讯作者：
Wu HG