Identification of soybean microRNAs involved in soybean cyst nematode infection by deep sequencing.
Identification of soybean microRNAs involved in soybean cyst nematode infection by deep sequencing.
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通过深度测序鉴定参与大豆胞囊线虫感染的大豆 MicroRNA
DOI：
10.1371/journal.pone.0039650
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Dong W
中科院分区：
文献类型：
--
作者：
Li X;Wang X;Zhang S;Liu D;Duan Y;Dong W
大豆胞囊线虫（Soybean cyst nematode，SCN）是世界范围内危害大豆最严重的病原菌。MicroRNAs（miRNAs）是一类在植物逆境应答中发挥重要作用的非编码小分子RNA。然而，有很少的报道，分析在病原体胁迫过程中的miRNA表达模式。对两个大豆品种（海宾小黑豆，抗SCN 3号小种，辽豆10号，感SCN 3号小种）在未接种和接种SCN的土壤中的4个小RNA文库进行了测序。将小分子RNA定位到大豆基因组序列中，共鉴定出364个已知的大豆miRNA基因。此外，还鉴定了21种潜在的miRNA候选物。miRNA谱的比较分析表明，来自40个家族的101个miRNA是SCN反应性的。另外，我们还发现了20个在同一品种的两个品种之间表达模式不同的miRNAs。这些结果表明，miRNA在大豆对SCN的反应中起重要作用，对进一步鉴定病原菌胁迫下的miRNA具有重要意义。
Soybean cyst nematode (SCN), Heterodera glycines, is the most devastating pathogen of soybean worldwide. MicroRNAs (miRNAs) are a class of small, non-coding RNAs that are known to play important role in plant stress response. However, there are few reports profiling the miRNA expression patterns during pathogen stress. We sequenced four small RNA libraries from two soybean cultivar (Hairbin xiaoheidou, SCN race 3 resistant, Liaodou 10, SCN race 3 susceptible) that grown under un-inoculated and SCN-inoculated soil. Small RNAs were mapped to soybean genome sequence, 364 known soybean miRNA genes were identified in total. In addition, 21 potential miRNA candidates were identified. Comparative analysis of miRNA profiling indicated 101 miRNAs belong to 40 families were SCN-responsive. We also found 20 miRNAs with different express pattern even between two cultivars of the same species. These findings suggest that miRNA paly important role in soybean response to SCN and have important implications for further identification of miRNAs under pathogen stress.
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影响因子：
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作者：
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通讯作者：
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作者：
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Mirbase：MicroRNA基因组学的工具。
影响因子：
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作者：
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通讯作者：
Enright, Anton J.
影响因子：
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作者：
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