Molecular characterization of a genomic interval with highly uneven recombination distribution on maize chromosome 10 L
Molecular characterization of a genomic interval with highly uneven recombination distribution on maize chromosome 10 L
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玉米 10 L 染色体上重组分布高度不均匀的基因组区间的分子表征
DOI：
10.1007/s10709-011-9613-x
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发表时间：
2011-11
期刊：
影响因子：
1.5
通讯作者：
Song, Rentao
中科院分区：
文献类型：
--
作者：
Zhou, Liangliang;Wang, Fei;Xu, Zhengkai;Song, Rentao
减数分裂中的同源重组通过产生遗传变异提供了真核生物进化的驱动力。减数分裂重组并不总是均匀地发生在染色体上，因此遗传距离和物理距离并不总是成比例的。通过对13,933个回交后代的分析，发现在第10号染色体长臂（10 L）上有一个278 kb的片段。重组事件在时间间隔内分布不均匀。遗传距离与物理距离的比值在这段时间内波动约47倍。在分子标记的辅助下，将该区间划分为若干个亚区间，以便进一步表征。与以前的观察结果一致，高基因密度区域，如子区间A和B也是遗传重组的热点子区间，而富含重复序列的区域，如子区间C也被发现是重组惰性的检测水平的研究。然而，我们发现了一个不寻常的子区间D，其中72-kb的区域包含6个基因。亚区间D的基因密度是全基因组平均值的5.8倍。亚区间D的遗传距离与物理距离之比为0.58cM/Mb，约为基因组平均值的3/4。我们对亚区间D的序列多态性和甲基化状态进行了分析，并讨论了重组抑制的潜在原因。这项研究是对玉米基因组中一个不寻常的重组区域进行详细遗传分析的另一个案例。
Homologous recombination in meiosis provides the evolutionary driving force in eukaryotic organisms by generating genetic variability. Meiotic recombination does not always occur evenly across the chromosome, and therefore genetic and physical distances are not consistently in proportion. We discovered a 278 kb interval on the long arm of chromosome 10 (10 L) through analyzed 13,933 descendants of backcross population. The recombinant events distributed unevenly in the interval. The ratio of genetic to physical distance in the interval fluctuated about 47-fold. With the assistance of molecular markers, the interval was divided into several subintervals for further characterization. In agreement with previous observations, high gene-density regions such as subinterval A and B were also genetic recombination hot subintervals, and repetitive sequence-riched region such as subinterval C was also found to be recombination inert at the detection level of the study. However, we found an unusual subinterval D, in which the 72-kb region contained 6 genes. The gene-density of subinterval D was 5.8 times that of the genome-wide average. The ratio of genetic to physical distance in subinterval D was 0.58 cM/Mb, only about 3/4 of the genome average. We carried out an analysis of sequence polymorphisms and methylation status in subinterval D, and the potential causes of recombination suppression were discussed. This study was another case of a detailed genetic analysis of an unusual recombination region in the maize genome.
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影响因子：
3.3
作者：
Hong Yao;P. Schnable
通讯作者：
Hong Yao;P. Schnable
DOI：
10.1073/pnas.132259199
发表时间：
2002-07-09
影响因子：
11.1
作者：
Fu, HH;Dooner, HK
通讯作者：
Dooner, HK
影响因子：
7
作者：
Song, RT;Llaca, V;Messing, J
通讯作者：
Messing, J
影响因子：
7.4
作者：
Haberer, G;Young, S;Messing, J
通讯作者：
Messing, J
影响因子：
10.5
作者：
Maloisel, L;Rossignol, JL
通讯作者：
Rossignol, JL