Reviewing the Effects of Skin Manipulations on Adult Newt Limb Regeneration: Implications for the Subcutaneous Origin of Axial Pattern Formation.
Reviewing the Effects of Skin Manipulations on Adult Newt Limb Regeneration: Implications for the Subcutaneous Origin of Axial Pattern Formation.
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DOI：
10.3390/biomedicines9101426
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发表时间：
2021-10-09
期刊：
影响因子：
4.7
通讯作者：
Chiba C
中科院分区：
文献类型：
--
作者：
Casco-Robles MM;Yasuda K;Yahata K;Maruo F;Chiba C
蝾螈是一种独特的火蜥蜴，可以在变性后的成体中再生四肢。为了像蝾螈一样再生人类的四肢，有必要确定那些与那些促进成年蝾螈肢体再生的细胞同源的细胞是否也存在于人类。以前对幼虫两栖动物的皮肤处理研究表明，残肢皮肤在再生肢体的轴向模式中起着关键作用。然而，尽管这类研究提供了丰富的信息，但在成年蝾螈身上的研究是有限的。因此，在本文中，我们进行了皮肤手法实验，如将皮肤绕肢体近端轴线旋转180°，并用肢体或身体上另一位置的皮肤替换一半皮肤。我们发现，与我们的预期相反，无论皮肤如何处理，成年蝾螈都能以正常的轴向模式健壮地再生四肢，并且异常的出现是随机的。我们的结果表明，在成人纽特肢体再生中，皮肤下的组织而不是皮肤本身在保证正常的轴型形成中起着首要的作用。我们认为重要的组织位于腹前和腹后皮肤下面的小区域。
Newts are unique salamanders that can regenerate their limbs as postmetamorphic adults. In order to regenerate human limbs as newts do, it is necessary to determine whether the cells homologous to those contributing to the limb regeneration of adult newts also exist in humans. Previous skin manipulation studies in larval amphibians have suggested that stump skin plays a pivotal role in the axial patterning of regenerating limbs. However, in adult newts such studies are limited, though they are informative. Therefore, in this article we have conducted skin manipulation experiments such as rotating the skin 180° around the proximodistal axis of the limb and replacing half of the skin with that of another location on the limb or body. We found that, contrary to our expectations, adult newts robustly regenerated limbs with a normal axial pattern regardless of skin manipulation, and that the appearance of abnormalities was stochastic. Our results suggest that the tissue under the skin, rather than the skin itself, in the intact limb is of primary importance in ensuring the normal axial pattern formation in adult newt limb regeneration. We propose that the important tissues are located in small areas underlying the ventral anterior and ventral posterior skin.
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从干细胞到肢体肌肉再生的开发调节转换。
影响因子：
16.6
作者：
Tanaka HV;Ng NCY;Yang Yu Z;Casco-Robles MM;Maruo F;Tsonis PA;Chiba C
通讯作者：
Chiba C
影响因子：
3.7
作者：
Nakamura K;Islam MR;Takayanagi M;Yasumuro H;Inami W;Kunahong A;Casco-Robles RM;Toyama F;Chiba C
通讯作者：
Chiba C
DOI：
10.1002/reg2.92
发表时间：
2017-08
期刊：
Regeneration (Oxford, England)
影响因子：
--
作者：
Stocum DL
通讯作者：
Stocum DL
影响因子：
14.8
作者：
Casco-Robles, Martin Miguel;Yamada, Shouta;Chiba, Chikafumi
通讯作者：
Chiba, Chikafumi
影响因子：
--
作者：
TANK, PW
通讯作者：
TANK, PW