CD8 T-cell activation in mice injected with a plasmid DNA vaccine encoding AMA-1 of the reemerging Korean Plasmodium vivax.
CD8 T-cell activation in mice injected with a plasmid DNA vaccine encoding AMA-1 of the reemerging Korean Plasmodium vivax.
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DOI：
10.3347/kjp.2011.49.1.85
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发表时间：
2011-03
期刊：
影响因子：
--
通讯作者：
Chai JY
中科院分区：
文献类型：
--
作者：
Kim HJ;Jung BK;Lee JJ;Pyo KH;Kim TY;Choi BI;Kim TW;Hisaeda H;Himeno K;Shin EH;Chai JY
针对间日疟原虫AMA-1疫苗和编码间日疟原虫AMA-1的质粒DNA疫苗（PvAMA-1）的研究相对较少。本研究构建了韩国间日疟原虫AMA-1编码的质粒DNA疫苗，并对其对BALB/c小鼠的细胞免疫原性进行了初步研究。将PvAMA-1基因克隆并在质粒载体UBpcAMA-1中表达，在转染的COS7细胞中获得约56.8 kDa的蛋白带。以BALB/c小鼠肌注或基因枪免疫4次，末次注射后第2周用荧光细胞法检测脾t细胞亚群比例。肌内注射小鼠脾细胞CD8+ t细胞和CD4+ t细胞的比例未见明显变化。然而，基因枪免疫小鼠的CD8+细胞比例明显高于注射UB载体的对照组（P<0.05）。结果表明，韩国间日疟AMA-1编码质粒DNA疫苗肌肉注射时细胞免疫原性较弱；然而，使用基因枪注射技术观察到有希望的效果。
Relatively little has been studied on the AMA-1 vaccine against Plasmodium vivax and on the plasmid DNA vaccine encoding P. vivax AMA-1 (PvAMA-1). In the present study, a plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax has been constructed and a preliminary study was done on its cellular immunogenicity to recipient BALB/c mice. The PvAMA-1 gene was cloned and expressed in the plasmid vector UBpcAMA-1, and a protein band of approximately 56.8 kDa was obtained from the transfected COS7 cells. BALB/c mice were immunized intramuscularly or using a gene gun 4 times with the vaccine, and the proportions of splenic T-cell subsets were examined by fluorocytometry at week 2 after the last injection. The spleen cells from intramuscularly injected mice revealed no significant changes in the proportions of CD8+ T-cells and CD4+ T-cells. However, in mice immunized using a gene gun, significantly higher (P<0.05) proportions of CD8+ cells were observed compared to UB vector-injected control mice. The results indicated that cellular immunogenicity of the plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax was weak when it was injected intramuscularly; however, a promising effect was observed using the gene gun injection technique.
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