Forelimb amputation-induced reorganization in the cuneate nucleus (CN) is not reflected in large-scale reorganization in rat forepaw barrel subfield cortex (FBS).
Forelimb amputation-induced reorganization in the cuneate nucleus (CN) is not reflected in large-scale reorganization in rat forepaw barrel subfield cortex (FBS).
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DOI：
10.1016/j.brainres.2013.05.049
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发表时间：
2013-08-14
期刊：
影响因子：
2.9
通讯作者：
Waters RS
中科院分区：
文献类型：
--
作者：
Li CX;Yang Q;Vemulapalli S;Waters RS
我们研究了重组在幼年大鼠的楔状核（CN）前肢截肢（n=34）和在完整的控制（n=5），以确定CN是否形成一个大规模的重组在前爪桶子区（FBS）皮质基板。来自肩部的新输入在截肢后4周首先出现在FBS中，并且到6周时，新的肩部输入开始占据FBS的大部分。电生理记录被用来映射CN在控制和前肢截肢者在第一个12周后去传入神经和截肢后26和30周。标测仅限于奥贝克斯前300 μm的位置，其中内侧至外侧的电极穿透穿过与无毛前爪趾和垫的代表相关的细胞色素氧化酶染色簇（称为barbettes）的完整补充，以及与腕部、手臂和肩部的代表相关的相邻非簇区域。截肢后，非群集区被来自身体/胸部和头部/颈部的新输入占据，而群集区除了边界外基本上没有新输入。一项回归分析比较了截肢后前12周内对照组和截肢者的情况，发现非集群区中身体/胸部和头部/颈部的新输入总面积存在显着差异，而任何新输入均未发现显着差异进入集群区。当重新检查身体部位代表的平均面积占平均区域面积的百分比时，在截肢后2-3周内观察到簇区内来自身体的新输入的非显著增加，并在随后的几周内恢复到基线。相比之下，在截肢后的前12周内，在非群集区发现身体/胸部和头部/颈部的身体部位表示的平均面积存在显着差异。目前的研究结果表明，重组只发生在非集群区，其中新的输入从身体/胸部和头部/颈部的移动，并立即截肢后占领的deafferented领土。此外，在截肢后的前12周内，在群集或非群集区中的新肩部输入缺乏显著差异，这表明CN为FBS中的大规模重组提供了不太可能的底物。
We examined reorganization in cuneate nucleus (CN) in juvenile rat following forelimb amputation (n=34) and in intact controls (n=5) to determine whether CN forms a substrate for large-scale reorganization in forepaw barrel subfield (FBS) cortex. New input from the shoulder first appears in the FBS 4 weeks after amputation, and by 6 weeks, the new shoulder input comes to occupy most of the FBS. Electrophysiological recording was used to map CN in controls and in forelimb amputees during the first 12 weeks following deafferentation and at 26 and 30 weeks post-amputation. Mapping was confined to a location 300 μm anterior to the obex where a medial-to-lateral row of electrode penetrations traversed through a complete complement of cytochrome-oxidase stained clusters (called barrelettes) that are associated with the representation of the glabrous forepaw digits and pads and adjacent non-cluster zones that are associated with the representation of the wrist, arm, and shoulder. Following amputation, non-cluster zones became occupied with new input from the body/chest and head/neck, while the cluster zone remained largely devoid of new input except at the border. A regression analysis comparing controls and amputees over the first 12 weeks post-amputation found significant differences for the total area of new input from the body/chest and head/neck in the non-cluster zones, while no significant differences were found for any new input into the cluster zone. When the averaged areas of a body-part representation were reexamined as a percentage of the averaged zonal area, a non-significant increase in new input from the body was observed within the cluster zone during post-amputation weeks 2–3 that returned to baseline in the subsequent weeks. In contrast, significant differences in averaged area of body-part representations for body/chest and head/neck were found in non-cluster zones over the first 12 weeks post-amputation. The present findings suggest that reorganization occurs only within the non-cluster zones whereby new input from the body/chest and head/neck moves in and occupies the deafferented territory immediately after amputation. Additionally, the lack of significant differences in new shoulder input in either cluster or non-cluster zones over the first 12 weeks after amputation suggests that CN provides an unlikely substrate for large-scale reorganization in the FBS.
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