Characterization of an IncFII plasmid encoding NDM-1 from Escherichia coli ST131.
Characterization of an IncFII plasmid encoding NDM-1 from Escherichia coli ST131.
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DOI：
10.1371/journal.pone.0034752
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Nordmann P
中科院分区：
文献类型：
--
作者：
Bonnin RA;Poirel L;Carattoli A;Nordmann P
目前编码金属内酰胺酶NDM-1的基因在肠杆菌科中的传播与周围的各种遗传结构和质粒支架有关。用高密度焦磷酸测序法测定了携带bla NDM-1基因的pGUE-NDM的全序列，并与其他bla NDM-1阴性的IncFII进行了基因组比较分析。在大肠杆菌中复制的pGUE-NDM对包括β-内酰胺类、氨基糖苷类、甲氧嘧啶和磺胺类药物在内的多种抗生素分子具有耐药性。它全长87,022个碱基，携带两个β-内酰胺酶基因bla NDM-1和bla OXA-1，以及三个氨基糖苷类耐药基因aacA4、aadA2和aacC2。对多重耐药基因的比较分析包含一个包含bla NDM-1基因的模块，该基因实际上在其他肠道细菌分离株的不同结构中是保守的。该模块由bla NDM-1基因、插入序列ISAba125和博莱霉素抗性编码基因组成。这是首次鉴定的携带IncFII型BLANDM-1的质粒。BLANDM-1基因和IncFII型质粒骨架之间的这种关联是非常令人担忧的，因为这种质粒型已知可以有效传播，例如BLACTX-M-15携带的IncFII质粒在世界范围内的传播。
The current spread of the gene encoding the metallo-ß-lactamase NDM-1 in Enterobacteriaceae is linked to a variety of surrounding genetic structures and plasmid scaffolds. The whole sequence of plasmid pGUE-NDM carrying the bla NDM-1 gene was determined by high-density pyrosequencing and a genomic comparative analysis with other bla NDM-1-negative IncFII was performed. Plasmid pGUE-NDM replicating in Escherichia coli confers resistance to many antibiotic molecules including β-lactams, aminoglycosides, trimethoprim, and sulfonamides. It is 87,022 bp in-size and carries the two β-lactamase genes bla NDM-1 and bla OXA-1, together with three aminoglycoside resistance genes aacA4, aadA2, and aacC2. Comparative analysis of the multidrug resistance locus contained a module encompassing the bla NDM-1 gene that is actually conserved among different structures identified in other enterobacterial isolates. This module was constituted by the bla NDM-1 gene, a fragment of insertion sequence ISAba125 and a bleomycin resistance encoding gene. This is the first characterized bla NDM-1-carrying IncFII-type plasmid. Such association between the bla NDM-1 gene and an IncFII-type plasmid backbone is extremely worrisome considering that this plasmid type is known to spread efficiently, as examplified with the worldwide dissemination of bla CTX-M-15-borne IncFII plasmids.
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