Prokaryotic expression of pathogenesis related protein 1 gene from Nicotiana benthamiana: antifungal activity and preparation of its polyclonal antibody

Prokaryotic expression of pathogenesis related protein 1 gene from Nicotiana benthamiana: antifungal activity and preparation of its polyclonal antibody
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本塞姆氏烟草病程相关蛋白1基因的原核表达:抗真菌活性及其多克隆抗体的制备

DOI:
10.1007/s10529-012-0851-5
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发表时间:
2012-01
影响因子:
2.7
通讯作者:
林宏辉
林宏辉
中科院分区:
工程技术4区
文献类型:
--
作者:
林宏辉

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利用RT-PCR技术从本氏烟草(Nicotianabenthamiana)中克隆了病程相关蛋白1(PR-1)基因的核苷酸序列。在PR-1的ORF片段上引入EcoRI和NotI的限制性内切酶位点,
The nucleotide sequence of the pathogenesis-related protein 1(PR-1) gene was obtained from Nicotiana benthamiana using RT-PCR. Restriction enzyme cutting sites of EcoRI and NotI were introduced to the ORF fragments of PR-1, they were then linked together
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