Abundantly Present miRNAs in Milk-Derived Extracellular Vesicles Are Conserved Between Mammals.
Abundantly Present miRNAs in Milk-Derived Extracellular Vesicles Are Conserved Between Mammals.
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DOI：
10.3389/fnut.2018.00081
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发表时间：
2018
影响因子：
5
通讯作者：
Wauben MHM
中科院分区：
文献类型：
--
作者：
van Herwijnen MJC;Driedonks TAP;Snoek BL;Kroon AMT;Kleinjan M;Jorritsma R;Pieterse CMJ;Hoen ENMN;Wauben MHM
哺乳动物的乳汁不仅是新生儿的营养来源，还含有调节进一步发育的各种成分。例如，牛奶是microRNA（miRNA）的丰富来源，microRNA是进化上保守的小的非编码RNA，参与靶mRNA的转录后调节。存在于牛奶中的miRNA可以存在于细胞外囊泡（EV）中，其是由许多细胞类型释放的纳米尺寸的膜囊泡，作为细胞间通讯的手段。EV的膜保护封闭的miRNA免于降解，并含有允许特异性靶向受体细胞的分子。虽然一些研究已经调查了来自单个物种的牛奶EV中的miRNA含量，但对不同物种之间EV相关miRNA的进化保守性知之甚少。在这项研究中，我们分析了从人乳和母乳中纯化的EV的miRNA含量。将这些数据与已发表的关于来自人类、牛、猪和熊猫乳汁的EV的研究进行比较，以评估前20种最丰富的miRNA的重叠。有趣的是，几种丰富的miRNA在物种之间共享（例如，let-7家族成员let-7a、let-7 b、let-7 f和miR-148 a）。此外，这些miRNAs与免疫相关的功能以及细胞生长和信号转导的调节有关。这些miRNA在物种之间的保守性，不仅在它们的序列同源性上，而且在它们在几个物种的乳汁EV中的掺入上，表明它们在进化上被选择来调节新生儿的细胞功能。
Mammalian milk is not only a source of nutrition for the newborn, but also contains various components that regulate further development. For instance, milk is an abundant source of microRNAs (miRNAs), which are evolutionary conserved small non-coding RNAs that are involved in post-transcriptional regulation of target mRNA. MiRNAs present in milk can occur in extracellular vesicles (EVs), which are nanosized membrane vesicles released by many cell types as a means of intercellular communication. The membrane of EVs protects enclosed miRNAs from degradation and harbors molecules that allow specific targeting to recipient cells. Although several studies have investigated the miRNA content in milk EVs from individual species, little is known about the evolutionary conserved nature of EV-associated miRNAs among different species. In this study, we profiled the miRNA content of purified EVs from human and porcine milk. These data were compared to published studies on EVs from human, cow, porcine, and panda milk to assess the overlap in the top 20 most abundant miRNAs. Interestingly, several abundant miRNAs were shared between species (e.g., let-7 family members let-7a, let-7b, let-7f, and miR-148a). Moreover, these miRNAs have been implicated in immune-related functions and regulation of cell growth and signal transduction. The conservation of these miRNA among species, not only in their sequence homology, but also in their incorporation in milk EVs of several species, suggests that they are evolutionarily selected to regulate cell function in the newborn.
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影响因子：
7.3
作者：
van der Grein SG;Nolte-'t Hoen EN
通讯作者：
Nolte-'t Hoen EN
影响因子：
16
作者：
Lötvall J;Hill AF;Hochberg F;Buzás EI;Di Vizio D;Gardiner C;Gho YS;Kurochkin IV;Mathivanan S;Quesenberry P;Sahoo S;Tahara H;Wauben MH;Witwer KW;Théry C
通讯作者：
Théry C
影响因子：
16
作者：
Blans K;Hansen MS;Sørensen LV;Hvam ML;Howard KA;Möller A;Wiking L;Larsen LB;Rasmussen JT
通讯作者：
Rasmussen JT
影响因子：
4.4
作者：
Modepalli V;Kumar A;Hinds LA;Sharp JA;Nicholas KR;Lefevre C
通讯作者：
Lefevre C
影响因子：
4.8
作者：
Patel, Vishal;Carrion, Katrina;Nigam, Vishal
通讯作者：
Nigam, Vishal