High throughput viscoelastic particle focusing and separation in spiral microchannels.
High throughput viscoelastic particle focusing and separation in spiral microchannels.
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DOI：
10.1038/s41598-021-88047-4
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发表时间：
2021-04-19
影响因子：
4.6
通讯作者：
Russom A
中科院分区：
文献类型：
--
作者：
Kumar T;Ramachandraiah H;Iyengar SN;Banerjee I;Mårtensson G;Russom A
近年来，使用惯性和弹惯性微流体的被动颗粒操纵引起了人们的极大兴趣，并在高通量颗粒分选和分离中找到了各种应用。对于分离应用，与惯性微流体相比，迄今为止，弹惯性微流体的流速要低得多。在这项工作中，我们探索了螺旋通道中粘弹性颗粒的聚焦和分离，雷诺数比之前报道的高两个数量级。我们表明，主导惯性升力、迪安阻力和弹力之间的平衡使得 3D 粒子能够在动态高雷诺数下聚焦。使用两匝螺旋，我们表明，最初使用弹性增强剂 PEO（聚环氧乙烷）作为护套将颗粒挤压向内壁，严格根据尺寸向外壁迁移，并且可以高精度地有效分离。作为高分辨率颗粒分离原理的证明，15 µm 颗粒与 10 µm 颗粒有效分离。 10 µm 颗粒的分离效率为 98%，15 µm 颗粒的分离效率为 97%。此外，我们使用新型集成双螺旋装置展示了无鞘、高通量分离，并在 1 mL/min 的样品流速下实现了 10 µm 颗粒 89% 和 15 µm 颗粒 99% 的分离效率——之前仅报道了惯性微流体的通量。我们预计，以极高的流速精确控制 3D 粒子的能力将开辟多种应用，包括开发超高通量微流式细胞仪和用于护理诊断的稀有细胞的高分辨率分离。
Passive particle manipulation using inertial and elasto-inertial microfluidics have received substantial interest in recent years and have found various applications in high throughput particle sorting and separation. For separation applications, elasto-inertial microfluidics has thus far been applied at substantial lower flow rates as compared to inertial microfluidics. In this work, we explore viscoelastic particle focusing and separation in spiral channels at two orders of magnitude higher Reynolds numbers than previously reported. We show that the balance between dominant inertial lift force, dean drag force and elastic force enables stable 3D particle focusing at dynamically high Reynolds numbers. Using a two-turn spiral, we show that particles, initially pinched towards the inner wall using an elasticity enhancer, PEO (polyethylene oxide), as sheath migrate towards the outer wall strictly based on size and can be effectively separated with high precision. As a proof of principle for high resolution particle separation, 15 µm particles were effectively separated from 10 µm particles. A separation efficiency of 98% for the 10 µm and 97% for the 15 µm particles was achieved. Furthermore, we demonstrate sheath-less, high throughput, separation using a novel integrated two-spiral device and achieved a separation efficiency of 89% for the 10 µm and 99% for the 15 µm particles at a sample flow rate of 1 mL/min—a throughput previously only reported for inertial microfluidics. We anticipate the ability to precisely control particles in 3D at extremely high flow rates will open up several applications, including the development of ultra-high throughput microflow cytometers and high-resolution separation of rare cells for point of care diagnostics.
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影响因子：
4.6
作者：
Etcheverry S;Faridi A;Ramachandraiah H;Kumar T;Margulis W;Laurell F;Russom A
通讯作者：
Russom A
影响因子：
6.1
作者：
D'Avino, Gaetano;Romeo, Giovanni;Maffettone, Pier Luca
通讯作者：
Maffettone, Pier Luca
影响因子：
9.3
作者：
Lin, Eric;Rivera-Baez, Lianette;Nagrath, Sunitha
通讯作者：
Nagrath, Sunitha
影响因子：
6.1
作者：
Kuntaegowdanahalli, Sathyakumar S.;Bhagat, Ali Asgar S.;Papautsky, Ian
通讯作者：
Papautsky, Ian
影响因子：
56.9
作者：
Huang, LR;Cox, EC;Sturm, JC
通讯作者：
Sturm, JC