Microsphere-based seamless scaffolds containing macroscopic gradients of encapsulated factors for tissue engineering.
Microsphere-based seamless scaffolds containing macroscopic gradients of encapsulated factors for tissue engineering.
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基于微球的无缝支架，其中包含组织工程封装因子的宏观梯度。
DOI：
10.1089/ten.tec.2008.0167
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发表时间：
2008-12
期刊：
影响因子：
--
通讯作者：
Berkland C
中科院分区：
文献类型：
--
作者：
Singh M;Morris CP;Ellis RJ;Detamore MS;Berkland C
在三维（3D）组织工程支架中的生物活性信号的空间和时间控制是非常需要的。结合在一起，这些属性可以模拟和维持复杂的信号模式，例如在轴突再生或新血管形成期间观察到的信号模式。无缝聚合物构建体可以提供实现信号分布的空间控制的途径。在这项研究中，引入了一种新的基于微粒的支架制造技术，作为一种方法来创建3D支架的空间控制模型染料使用均匀的聚（D，L-丙交酯-共-乙交酯）微球。使用精密颗粒制造技术生产均匀的微球。通过将微球悬浮液流入圆柱形玻璃模具中来组装支架，然后使用乙醇处理将微球物理附着以形成连续支架。发现1小时的乙醇浸泡对于改善机械特性是最佳的。支架的形态学和物理特性表明，微球基质是多孔的（41.1 ± 2.1%），连接良好，其压缩刚度范围为142至306 kPa。在支架上培养软骨细胞揭示了这些基质与细胞附着和活力的相容性。此外，双层，多层和梯度支架的制造，表现出良好的空间控制和分辨率。这种新型的支架可以作为持续传递装置的异质信号在一个连续和无缝的方式，并可能是特别有用的，在未来的界面组织工程研究。
Spatial and temporal control of bioactive signals in three-dimensional (3D) tissue engineering scaffolds is greatly desired. Coupled together, these attributes may mimic and maintain complex signal patterns, such as those observed during axonal regeneration or neovascularization. Seamless polymer constructs may provide a route to achieve spatial control of signal distribution. In this study, a novel microparticle-based scaffold fabrication technique is introduced as a method to create 3D scaffolds with spatial control over model dyes using uniform poly(D,L-lactide-co-glycolide) microspheres. Uniform microspheres were produced using the Precision Particle Fabrication technique. Scaffolds were assembled by flowing microsphere suspensions into a cylindrical glass mold, and then microspheres were physically attached to form a continuous scaffold using ethanol treatment. An ethanol soak of 1 h was found to be optimum for improved mechanical characteristics. Morphological and physical characterization of the scaffolds revealed that microsphere matrices were porous (41.1 ± 2.1%) and well connected, and their compressive stiffness ranged from 142 to 306 kPa. Culturing chondrocytes on the scaffolds revealed the compatibility of these substrates with cell attachment and viability. In addition, bilayered, multilayered, and gradient scaffolds were fabricated, exhibiting excellent spatial control and resolution. Such novel scaffolds can serve as sustained delivery devices of heterogeneous signals in a continuous and seamless manner, and may be particularly useful in future interfacial tissue engineering investigations.
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影响因子：
10.8
作者：
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