Ghrelin is produced in taste cells and ghrelin receptor null mice show reduced taste responsivity to salty (NaCl) and sour (citric acid) tastants.
Ghrelin is produced in taste cells and ghrelin receptor null mice show reduced taste responsivity to salty (NaCl) and sour (citric acid) tastants.
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DOI：
10.1371/journal.pone.0012729
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发表时间：
2010-09-14
期刊：
影响因子：
3.7
通讯作者：
Egan JM
中科院分区：
文献类型：
--
作者：
Shin YK;Martin B;Kim W;White CM;Ji S;Sun Y;Smith RG;Sévigny J;Tschöp MH;Maudsley S;Egan JM
味觉系统在决定食物偏好、食物摄入和能量平衡方面起着关键作用。精确的机制，微调味觉敏感性目前还不清楚，但很明显，许多因素，如传出输入和特定的信号转导级联。使用免疫组织化学分析，我们表明，饥饿素，一种激素，传统上被认为是一种食欲调节激素，存在于舌头的味蕾。前生长素释放肽原、激素原转化酶1/3（PC 1/3）、生长素释放肽及其同源受体（GHSR）和生长素释放肽-O-酰基转移酶（GOAT，激活生长素释放肽的酶）在小鼠味蕾的I、II、III和IV型味觉细胞中表达。此外，ghrelin和GHSR共定位于相同的味觉细胞中，表明ghrelin在味觉细胞中以自分泌方式起作用。为了确定生长激素释放肽在改变味觉感知中的作用，我们使用GHSR缺失小鼠进行味觉行为测试。GHSR缺失小鼠对酸味（柠檬酸）和咸味（氯化钠）促味剂的味觉反应显著降低。这些研究结果表明，ghrelin发挥了局部调节作用，在确定味蕾信号和功能，并可能是一种新的机制，调节咸和酸味的反应。
The gustatory system plays a critical role in determining food preferences, food intake and energy balance. The exact mechanisms that fine tune taste sensitivity are currently poorly defined, but it is clear that numerous factors such as efferent input and specific signal transduction cascades are involved. Using immunohistochemical analyses, we show that ghrelin, a hormone classically considered to be an appetite-regulating hormone, is present within the taste buds of the tongue. Prepro-ghrelin, prohormone convertase 1/3 (PC 1/3), ghrelin, its cognate receptor (GHSR), and ghrelin-O-acyltransferase (GOAT , the enzyme that activates ghrelin) are expressed in Type I, II, III and IV taste cells of mouse taste buds. In addition, ghrelin and GHSR co-localize in the same taste cells, suggesting that ghrelin works in an autocrine manner in taste cells. To determine a role for ghrelin in modifying taste perception, we performed taste behavioral tests using GHSR null mice. GHSR null mice exhibited significantly reduced taste responsivity to sour (citric acid) and salty (sodium chloride) tastants. These findings suggest that ghrelin plays a local modulatory role in determining taste bud signaling and function and could be a novel mechanism for the modulation of salty and sour taste responsivity.
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