An OMV-Based Nanovaccine Confers Safety and Protection against Pathogenic Escherichia coli via Both Humoral and Predominantly Th1 Immune Responses in Poultry.
An OMV-Based Nanovaccine Confers Safety and Protection against Pathogenic Escherichia coli via Both Humoral and Predominantly Th1 Immune Responses in Poultry.
复制标题
基于 OMV 的纳米疫苗通过家禽的体液免疫反应和以 Th1 为主的免疫反应，提供安全性和针对致病性大肠杆菌的保护。
DOI：
10.3390/nano10112293
复制
发表时间：
2020-11-20
期刊：
影响因子：
--
通讯作者：
Yang M
中科院分区：
文献类型：
--
作者：
Hu R;Liu H;Wang M;Li J;Lin H;Liang M;Gao Y;Yang M
禽致病性大肠杆菌（APEC）感染给家禽造成了巨大的经济损失和公共卫生风险。细菌外膜囊泡（OMV）和富含各种免疫原性分子的纳米级蛋白脂质作为针对细菌感染的新型纳米疫苗获得了广泛的兴趣。在本研究中，使用超速离心法制备APEC O2衍生的OMV（APEC_OMV）并使用电子显微镜和纳米颗粒追踪分析对其进行表征后，我们检查了APEC_OMV在肉鸡中的安全性和疫苗接种效果，并研究了潜在的免疫保护机制。结果表明，APEC_OMV具有膜包封结构，平均直径为89 nm。用50 μg的APC_OMV免疫雏鸡，无副作用，能有效地保护雏鸡免受同源性感染。体外实验表明，APEC_OMV能被鸡巨噬细胞有效摄取，并激活巨噬细胞的天然免疫反应。APEC_OMV疫苗能显著提高血清非特异性免疫因子的活性，增强特异性抗体反应，促进脾和外周血淋巴细胞对丝裂原的增殖反应。此外，APEC_OMV还引起脾淋巴细胞主要由IFN-γ介导的Th 1应答。我们的数据揭示了APEC_OMV介导的保护中涉及非特异性免疫应答和特异性抗体和细胞因子应答，为未来开发具有高安全性和有效性的多价APEC_OMV纳米疫苗提供了更广泛的知识。
Avian pathogenic Escherichia coli (APEC) infection in poultry causes enormous economic losses and public health risks. Bacterial outer membrane vesicles (OMVs) and nano-sized proteolipids enriched with various immunogenic molecules have gained extensive interest as novel nanovaccines against bacterial infections. In this study, after the preparation of APEC O2-derived OMVs (APEC_OMVs) using the ultracentrifugation method and characterization of them using electron microscopy and nanoparticle tracking analyses, we examined the safety and vaccination effect of APEC_OMVs in broiler chicks and investigated the underlying immunological mechanism of protection. The results showed that APEC_OMVs had membrane-enclosed structures with an average diameter of 89 nm. Vaccination with 50 μg of APEC_OMVs had no side effects and efficiently protected chicks against homologous infection. APEC_OMVs could be effectively taken up by chicken macrophages and activated innate immune responses in macrophages in vitro. APEC_OMV vaccination significantly improved activities of serum non-specific immune factors, enhanced the specific antibody response and promoted the proliferation of splenic and peripheral blood lymphocytes in response to mitogen. Furthermore, APEC_OMVs also elicited a predominantly IFN-γ-mediated Th1 response in splenic lymphocytes. Our data revealed the involvement of both non-specific immune responses and specific antibody and cytokine responses in the APEC_OMV-mediated protection, providing broader knowledge for the development of multivalent APEC_OMV-based nanovaccine with high safety and efficacy in the future.
登录
查看更多内容
DOI：
10.1038/nrmicro3480
发表时间：
2015-10
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Brown L;Wolf JM;Prados-Rosales R;Casadevall A
通讯作者：
Casadevall A
影响因子：
4.4
作者：
Avalos-Gomez, Christian;Reyes-Lopez, Magda;de la Garza, Mireya
通讯作者：
de la Garza, Mireya
影响因子：
4.4
作者：
Hoelzer K;Bielke L;Blake DP;Cox E;Cutting SM;Devriendt B;Erlacher-Vindel E;Goossens E;Karaca K;Lemiere S;Metzner M;Raicek M;Collell Suriñach M;Wong NM;Gay C;Van Immerseel F
通讯作者：
Van Immerseel F
利用大肠杆菌来源的外膜囊泡作为抗原递送平台可引发针对鲍曼不动杆菌感染的保护性免疫
影响因子：
4.6
作者：
Huang W;Wang S;Yao Y;Xia Y;Yang X;Li K;Sun P;Liu C;Sun W;Bai H;Chu X;Li Y;Ma Y
通讯作者：
Ma Y
影响因子：
3.7
作者：
Ebrahimi-Nik H;Bassami MR;Mohri M;Rad M;Khan MI
通讯作者：
Khan MI