Distribution of the octopamine receptor AmOA1 in the honey bee brain.
Distribution of the octopamine receptor AmOA1 in the honey bee brain.
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DOI：
10.1371/journal.pone.0014536
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发表时间：
2011-01-18
期刊：
影响因子：
3.7
通讯作者：
Smith BH
中科院分区：
文献类型：
--
作者：
Sinakevitch I;Mustard JA;Smith BH
章鱼胺在无脊椎动物的许多行为中起着重要作用。它通过与位于应答细胞质膜上的G蛋白偶联受体结合而起作用。在无脊椎动物中发现了几种不同的章鱼胺受体亚型，但对这些不同受体亚型的表达模式以及每种亚型如何影响不同的行为知之甚少。蜜蜂（Apis mellifera）的章鱼胺受体AmOA 1最近被克隆和鉴定。在这里，我们继续通过调查其分布在蜜蜂大脑的AmOA 1受体的特点。我们使用了两个独立的抗体对两个不同的肽在羧基末端研究蜜蜂大脑中的AmOA 1受体的分布。我们发现，这两种抗AmOA 1抗体揭示了整个大脑和以下脑神经细胞内的细胞体簇的标记：触角叶;花萼，花梗，垂直（α，γ）和内侧（β）叶的蘑菇体;视叶;食管下神经节;和中央复合体。使用抗GABA和抗AmOA 1受体抗体的双重免疫荧光染色显示，在触角叶中的抑制性GABA能局部中间神经元的群体在肾小球中的细胞体、轴突及其末梢中表达AmOA 1受体。在蘑菇体中，AmOA 1受体在抑制性GABA能反馈神经元的亚群中表达，该亚群终止于视觉（基底环和颈区的外半部）和嗅觉（唇和内基底环区）萼神经末梢，以及垂直和内侧叶的颈区和唇区。这些数据表明，章鱼胺通过AmOA 1在触角叶和蘑菇体中的作用之一是调节抑制性神经元。
Octopamine plays an important role in many behaviors in invertebrates. It acts via binding to G protein coupled receptors located on the plasma membrane of responsive cells. Several distinct subtypes of octopamine receptors have been found in invertebrates, yet little is known about the expression pattern of these different receptor subtypes and how each subtype may contribute to different behaviors. One honey bee (Apis mellifera) octopamine receptor, AmOA1, was recently cloned and characterized. Here we continue to characterize the AmOA1 receptor by investigating its distribution in the honey bee brain. We used two independent antibodies produced against two distinct peptides in the carboxyl-terminus to study the distribution of the AmOA1 receptor in the honey bee brain. We found that both anti-AmOA1 antibodies revealed labeling of cell body clusters throughout the brain and within the following brain neuropils: the antennal lobes; the calyces, pedunculus, vertical (alpha, gamma) and medial (beta) lobes of the mushroom body; the optic lobes; the subesophageal ganglion; and the central complex. Double immunofluorescence staining using anti-GABA and anti-AmOA1 receptor antibodies revealed that a population of inhibitory GABAergic local interneurons in the antennal lobes express the AmOA1 receptor in the cell bodies, axons and their endings in the glomeruli. In the mushroom bodies, AmOA1 receptors are expressed in a subpopulation of inhibitory GABAergic feedback neurons that ends in the visual (outer half of basal ring and collar regions) and olfactory (lip and inner basal ring region) calyx neuropils, as well as in the collar and lip zones of the vertical and medial lobes. The data suggest that one effect of octopamine via AmOA1 in the antennal lobe and mushroom body is to modulate inhibitory neurons.
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